Hericium Erinaceus HE015 Strain Breeding for Yield and Contaminant Resistance

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Solution Overview

Problem

Hericium erinaceus strains face issues of low genetic diversity, low yield, and susceptibility to living contaminants, leading to economic losses in cultivation and inadequate quality in existing varieties, failing to meet the demand for high-quality products in the deep processing industry.

Innovation Solution

A new Hericium erinaceus strain HE015 is developed through systematic breeding from wild samples, characterized by dense mycelia, fast growth, high yield, and resistance to living contaminants, along with a specific molecular marker for identification and extraction methods to enhance quality control.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional mutation breeding and crossbreeding methods are used to develop new Hericium erinaceus varieties, then new varieties can be obtained, but the genetic background remains similar and the varieties show little improvement in quality and yield

Engineering Contradiction:
Improvegenetic diversityVSAvoidyield and quality improvement
Core Design Contradiction:
Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent introduces wild Hericium erinaceus strains as an intermediary source of novel genetic material. These wild strains, collected from natural habitats, serve as a bridge between traditional cultivation and genetic improvement, providing diverse genetic backgrounds that differ significantly from conventional cultivated varieties. This intermediary approach enables introgression of wild genetic traits into cultivated strains through hybridization, thereby achieving both genetic diversity and improved productivity.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent transitions from traditional one-dimensional breeding (mutation and crossbreeding within cultivated varieties) to a multi-dimensional approach by incorporating wild strains from diverse geographic sources. This adds a new dimension of genetic variation, allowing breeders to access novel alleles and genetic combinations that were previously unavailable, thus simultaneously improving genetic diversity and agricultural traits.

Inventive Principle:
Principle #17Another dimension (Dimensionality change)

2Productivity

If breeding focuses on high yield characteristics, then yield increases, but quality deteriorates; conversely, if breeding focuses on fine quality, then quality improves, but yield decreases

Engineering Contradiction:
ImproveyieldVSAvoidquality
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent utilizes parameter changes in the genetic composition by introducing wild strain genes that carry different allele combinations. These genetic parameter changes result in novel phenotypic expressions where both yield and quality traits are improved simultaneously, breaking the traditional trade-off relationship. The wild genetic material provides new genetic variants that allow optimization of multiple traits without the conventional compromise.

Inventive Principle:
Principle #35Parameter changes

3Productivity

If conventional cultivated strains are used for scale production, then production can be maintained, but susceptibility to living contaminants increases causing economic losses

Engineering Contradiction:
Improveproduction stabilityVSAvoidresistance to living contaminants
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent segments the genetic background into cultivated and wild components, creating hybrid strains that combine the production stability of cultivated varieties with the contaminant resistance of wild strains. This genetic segmentation allows the offspring to inherit beneficial traits from both parents, achieving both production stability and enhanced reliability against living contaminants.

Inventive Principle:
Principle #1Segmentation

4Productivity

If existing Hericium erinaceus varieties are cultivated, then production can proceed, but the varieties fail to meet the increasing demand for high-quality products in deep processing industry

Engineering Contradiction:
Improveproduction volumeVSAvoidproduct quality for deep processing
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent develops hybrid strains with multi-functional characteristics that make them suitable for both traditional cultivation and deep processing applications. The strains exhibit universal adaptability, producing high-quality fruiting bodies with superior nutritional and functional properties that meet the diverse requirements of both fresh market and deep processing industry, thereby expanding the applicability and market value of the cultivated varieties.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Data Source

PatentEP4650434A1New hericium erinaceus he015 strain of hericium erinaceus, and molecular marker and use thereof
Publication Date: 2025.11.19 INFINITUS (CHINA) CO LTD
  • EP4650434A1 patent drawingFigure 1~2
  • EP4650434A1 patent drawingFigure 3~4B
  • EP4650434A1 patent drawingFigure 5~6

AI summary

The present invention discloses a new Hericium erinaceus strain HE015, and a molecular marker and use thereof. According to the present invention, wild fungi are widely collected throughout China and subjected to systematic strain breeding to obtain a new fine-quality Hericium erinaceus strain HE015. The strain HE015 was preserved in Guangdong Microbial Culture Collection Center on May 12, 2022 with an accession number of GDMCC No: 62464. The genetic background of the strain is obviously different from that of the existing Hericium erinaceus species; therefore, the new strain is of important value and significance in supplementing fine-quality cultivated Hericium erinaceus varieties, meeting the market demand and promoting high-quality development of the industry. The present invention further provides a specific molecular marker and a rapid detection method for detecting and identifying the strain HE015. The strain HE015 has advantages such as dense and sturdy mycelia of its mother strain, fast growth of its stock culture, strong living contaminants resistance, high yield and short growth cycle, and meanwhile contains high content of polysaccharide and other active ingredients. Its extract can protect gastric mucosa and improve alcohol-induced gastric mucosal damage. Particularly, its fruiting body extract has a more significant effect. Therefore, the strain of the present invention has a broad prospect of industrialization.