Mouse Model Preparation Using HSV-1 and Hydrocortisone
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Solution Overview
Problem
Current methods for developing animal models of herpes simplex virus infection do not effectively produce clear herpes lesions around the lips, limiting their usefulness for vaccine development and therapeutic agent testing.
Innovation Solution
A method involving the use of herpes simplex virus type 1 (HSV-1) and hydrocortisone to prepare a mouse model of herpes, where the skin is damaged, inoculated with HSV-1, and then treated with hydrocortisone to induce clear herpes lesions around the lips.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional methods are used to prepare animal models of herpes simplex virus infection, then the model can be established, but clear herpes lesions around the lips do not appear
Solution Approach 1:
Hydrocortisone is introduced as an intermediary substance to facilitate the appearance of clear herpes lesions. The steroid hormone mediates between the HSV-1 infection and the desired clinical manifestation, enabling the model to display characteristic lip lesions that were previously absent in conventional models
Solution Approach 2:
The immune status parameter of the animal model is changed through hydrocortisone administration. This parameter modification creates conditions favorable for herpes lesion development, transforming the model from one that fails to show lesions to one that clearly displays them around the lips
2Manufacturing precision
If the immune status is lowered to promote herpes lesion development, then lesions appear more clearly, but the animal's health and welfare may be compromised
Solution Approach 1:
Instead of completely suppressing the immune system, partial immunosuppression is achieved through hydrocortisone administration. This partial action is sufficient to promote herpes lesion development while avoiding the severe health consequences of complete immune suppression, maintaining animal welfare
3Ease of manufacture
If no adjuvant is used in the inoculation process, then the procedure is simpler, but herpes lesions do not develop clearly
Solution Approach 1:
Hydrocortisone serves as a chemical intermediary that bridges the gap between simple inoculation and clear lesion development. Rather than complicating the inoculation procedure with multiple steps or substances, a single hydrocortisone injection facilitates the entire lesion formation process
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method successfully creates a mouse model with clearly visible herpes lesions, enhancing its utility for developing therapeutic agents against herpes simplex virus infection by providing a more accurate representation of the disease.
Implementation Method 1
injecting the reared mouse with hydrocortisone
Data Source
AI summary
A method of preparing a mouse model of herpes using a herpes simplex virus and hydrocortisone, and a mouse model of herpes prepared using the method are disclosed. The method makes it possible to obtain an animal model in which symptoms of herpes clearly appear. The obtained animal model can be widely used in the development of therapeutic agents for herpes simplex virus infection. A use of the mouse model in screening and developing a candidate therapeutic agent is also disclosed.