Heteroaromatic Compounds for Stable Nucleic Acid Detection
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Solution Overview
Problem
Nucleic acid samples are prone to damage and degradation during sequencing due to environmental factors such as laser irradiation and EM radiation, leading to increased sequencing errors and loss of signal intensity.
Innovation Solution
The use of heteroaromatic ring compounds as nucleic acid protective agents, which are water-soluble, inert, and capable of quenching free radicals and absorbing high-energy radiation, thereby protecting nucleic acids during processing and detection.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If antioxidants (vitamin C derivatives and polyphenolic compounds) are used to protect nucleic acids from light-induced degradation, then nucleic acid stability is improved, but the antioxidants themselves degrade and deteriorate due to high reactivity with oxygen in air during storage and use
Solution Approach 1:
The patent employs heteroaromatic ring compounds as antioxidants that are stable during storage and use, replacing the conventional approach of using reactive antioxidants that degrade over time. These compounds provide sustained protection without self-degradation, effectively solving the reliability issue of antioxidant stability while maintaining nucleic acid protection.
2Stability of the object's composition
If polyphenolic compounds are used as antioxidants, then nucleic acid protection is achieved, but water solubility is poor limiting their use in aqueous systems
Solution Approach 1:
The patent utilizes heteroaromatic ring compounds with specific molecular structures that inherently possess good water solubility while maintaining antioxidant activity. By selecting compounds with appropriate hydrophilic groups and molecular properties, the invention achieves both nucleic acid protection and compatibility with aqueous sequencing systems, eliminating the solubility limitation of polyphenolic compounds.
3Object-affected harmful factors
If conventional antioxidants are used, then free radical quenching is achieved, but they react with oxygen in air during storage leading to degradation
Solution Approach 1:
The patent employs heteroaromatic ring compounds as antioxidants that are stable during storage and use, replacing the conventional approach of using reactive antioxidants that degrade over time. These compounds provide sustained protection without self-degradation, effectively solving the reliability issue of antioxidant stability while maintaining nucleic acid protection.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The heteroaromatic ring compounds effectively reduce nucleic acid damage, maintaining sample integrity and reducing sequencing errors while preserving fluorescence signals.
Implementation Method 1
The heteroaromatic ring compound...achieve the effect of protecting the nucleic acid sample by quenching free radicals and other harmful factors in the sample environment
Implementation Method 2
The heteroaromatic ring compound...absorbing strong EM radiation with short wavelength and high energy
Data Source
AI summary
Provided is the use of heteroaromatic ring compounds in nucleic acid detection. Particularly, provided are the use of heteroaromatic ring compounds as a nucleic acid protective agent in nucleic acid detection, a method for inhibiting degradation of nucleic acids during nucleic acid detection, a method for detecting a target nucleic acid molecule, and a method for determining a target single-stranded polynucleotide sequence. The present invention further relates to a reagent and a kit containing the heteroaromatic ring compounds.