HGF-Modified Odontogenic Stem Cells for Periodontal Tissue Repair
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Solution Overview
Problem
Current treatments for periodontal disease and bone tissue damage, such as periodontitis and fractures, face challenges in effectively repairing and regenerating periodontal and bone tissues due to limitations in stem cell survival and functionality post-implantation, with existing methods often resulting in high cell death rates and inadequate tissue repair.
Innovation Solution
Gene-modified odontogenic stem cells, specifically those expressing exogenous hepatocyte growth factor (HGF), are used to enhance tissue repair by promoting angiogenesis, reducing inflammation, and improving cell survival and proliferation, utilizing adenovirus or adeno-associated virus vectors for transfection, and administered as a cell suspension to target damaged areas.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional stem cell transplantation is used for periodontal tissue repair, then tissue regeneration is attempted, but cell survival and functionality post-implantation are limited resulting in high cell death rates
Solution Approach 1:
The patent modifies the genetic parameters of odontogenic stem cells by introducing exogenous HGF gene through viral vectors (adenovirus or adeno-associated virus). This genetic parameter change enables the cells to autonomously produce hepatocyte growth factor, transforming them from passive transplant cells into active therapeutic agents that secrete regenerative factors, thereby significantly improving both cell survival rate and tissue repair efficiency
Solution Approach 2:
The modified odontogenic stem cells are engineered to self-produce hepatocyte growth factor (HGF) through endogenous gene expression. This self-service mechanism eliminates the need for external HGF supplementation and allows the transplanted cells to autonomously create a pro-regenerative microenvironment, sustaining their own survival and promoting continuous tissue repair
2Ease of repair
If stem cells are transplanted to promote tissue regeneration, then repair capability is enhanced, but inflammation persists and cell functionality is compromised
Solution Approach 1:
The patent introduces hepatocyte growth factor (HGF) as a molecular intermediary between the transplanted odontogenic stem cells and the damaged periodontal tissue. HGF acts as a mediator that modulates the inflammatory response, suppressing harmful inflammation while promoting constructive repair processes, thereby resolving the contradiction between enhancing repair capability and reducing inflammation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of HGF-modified odontogenic stem cells significantly improves tissue repair by maintaining cell viability, reducing inflammation, and promoting tissue regeneration, demonstrating superior therapeutic effects compared to unmodified stem cells in treating periodontal and bone tissue defects.
Implementation Method 1
utilizing adenovirus or adeno-associated virus vectors for transfection
Implementation Method 2
promoting angiogenesis, reducing inflammation, and improving cell survival and proliferation
Implementation Method 3
maintaining cell viability, reducing inflammation, and promoting tissue regeneration
Data Source
Figure 1A~1B
Figure 2A~2O
Figure 3
AI summary
Provided in the present invention are odontogenic stem cells and a use of genetically modified odontogenic stem cells for treating periodontal disease, repairing defects in periodontal bone tissues or soft tissues and/or promoting the regeneration of periodontal tissues, or in products for treating acute and chronic bone tissue injuries (e.g. bone fracture) or bone tissue defects. Also provided in the present invention is a composition comprising odontogenic stem cells and/or genetically modified odontogenic stem cells, wherein an exogenous hepatocyte growth factor gene is introduced into odontogenic stem cells through an adenovirus or adeno-associated virus vector to obtain the genetically modified odontogenic stem cells.