High-Pressure Microbial Separation for Accurate Deep-Sea Detection
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Solution Overview
Problem
Current methods for separating and detecting marine microorganisms under high-pressure conditions lead to inaccurate results due to pressure changes and fail to maintain the original environmental conditions, limiting the detection of indigenous deep-sea microorganisms.
Innovation Solution
A continuous separation apparatus and method that includes a microorganism enrichment unit, gas injection unit, multi-sequence nutrient solution supply, multi-stage separation unit, and monitoring and cultivation unit, allowing for continuous separation and detection of marine microorganisms under high-pressure conditions, ensuring consistency with in situ marine pressures and environments.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If marine microorganisms are separated and detected after pressure release, then the detection process can be performed, but the detection results become inaccurate due to pressure changes
Solution Approach 1:
The patent employs a high-pressure inert environment (high-pressure nitrogen or carbon dioxide atmosphere) to maintain the original pressure conditions during the entire separation and detection process. This inert high-pressure environment prevents pressure-induced changes in microbial characteristics, allowing accurate detection of marine microorganisms without the harmful effect of pressure release.
Solution Approach 2:
The patent introduces a high-pressure inert gas (nitrogen or carbon dioxide) as an intermediary medium to maintain the pressure environment throughout the separation and detection process. This intermediary substance allows the system to isolate the microbial samples from atmospheric pressure changes while maintaining stable high-pressure conditions, thereby preventing pressure-induced detection errors.
2Reliability
If enrichment culture is conducted under high-pressure conditions, then original indigenous microorganisms can be obtained, but separation and detection become complex requiring pressure maintenance
Solution Approach 1:
The patent merges the enrichment culture, separation, and detection processes into a single integrated system that maintains high-pressure conditions throughout. By combining these functions in one continuous process rather than separate steps, the system achieves reliable acquisition of original indigenous microorganisms while avoiding the increased complexity of multiple pressure-controlled stages.
Solution Approach 2:
The patent designs a universal high-pressure inert atmosphere system that serves multiple functions: maintaining pressure during enrichment culture, enabling separation of microorganisms, and facilitating detection. This multi-functional approach achieves reliable microorganism acquisition while reducing overall system complexity by using a single pressure-maintenance mechanism for all purposes.
3Ease of operation
If environmental pressure is changed for detection, then detection can be performed, but microbial characteristics deviate from actual environment
Solution Approach 1:
The patent uses a high-pressure inert atmosphere as the detection environment, replacing atmospheric pressure with a controlled high-pressure inert gas environment. This maintains the original pressure conditions during detection, ensuring microbial characteristics remain accurate and consistent with their actual high-pressure marine habitat, while still enabling feasible detection operations.
Solution Approach 2:
The patent changes the detection parameters by conducting all separation and detection operations under maintained high-pressure conditions rather than releasing to atmospheric pressure. This parameter change (maintaining high pressure throughout) ensures microbial characteristics detected are accurate representations of their in-situ state, eliminating deviations caused by pressure changes.
Data Source
AI summary
The present invention discloses a continuous separation apparatus and method for marine microorganisms under high-pressure conditions. A microorganism enrichment unit is provided for microbial enrichment; a multi-sequence nutrient solution supply unit is used to supply a nutrient solution required for cultivation; a gas injection unit is used to supply culture gas or inert gas required for cultivation; and a pressure within the microorganism enrichment unit is enabled to be consistent with pressure of an in situ marine where the microorganisms is located; after a period of enrichment culture, a microbial liquid is pumped into a multi-stage separation unit to achieve multi-stage separation of different-size cells of the microbial liquid, thereby obtaining target microbial cells; and finally, the target microbial cells are transferred to a monitoring and cultivation unit for cultivation and stabilization, and an external multi-omics unit is used to perform cell characteristic detection using an external multi-omics unit.