High-Pressure Microbial Enrichment With Spray-Bead Isolation
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Solution Overview
Problem
Existing microbial enrichment and isolation technologies are primarily conducted in atmospheric environments, lacking the capability to simulate high-pressure conditions, resulting in low success rates for cultivating marine microorganisms, with less than 10% being successfully cultured, which hinders understanding of their phenotype, genes, and functional development.
Innovation Solution
A high-pressure environment biological enrichment and spray-type solid isolation and cultivation device that includes a spray-type isolation and cultivation unit, biological enrichment unit, pressurization system, temperature control system, and control-acquisition terminal, allowing for in-situ enrichment and isolation of marine microorganisms under simulated deep-sea conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Device complexity
If microbial enrichment and isolation are conducted in atmospheric environment, then the equipment complexity is reduced, but the cultivability of marine microorganisms deteriorates (less than 10% success rate)
Solution Approach 1:
The patent changes the environmental parameters from atmospheric conditions to high-pressure conditions (simulating deep-sea environment). The pressurization system maintains pressure levels consistent with deep-sea habitats, enabling marine microorganisms to be cultivated successfully under their natural environmental conditions, thereby improving cultivability from less than 10% to significantly higher rates
Solution Approach 2:
The patent introduces a high-pressure simulation system as an intermediary between the atmospheric laboratory environment and the deep-sea microbial habitat. This intermediary system (pressurization system, high-pressure reactor) allows researchers to replicate deep-sea conditions in the lab, serving as a bridge that enables successful cultivation of marine microorganisms without requiring actual deep-sea deployment
2Reliability
If high-pressure environment simulation is implemented, then the cultivability of marine microorganisms is improved, but the device complexity increases
Solution Approach 1:
The patent divides the cultivation system into separate functional modules: a pressurization system, a high-pressure reactor for enrichment, and a spray-type solid isolation unit. This segmentation allows each component to perform its specific function independently, making the overall complex system more manageable and easier to operate while maintaining high-pressure environment simulation capabilities
Solution Approach 2:
The patent employs pneumatic and hydraulic principles in the spray-type solid isolation unit, where liquid culture medium is sprayed onto solid support materials using pressure-driven nozzles. This approach simplifies the isolation process by using fluid dynamics to distribute nutrients and facilitate microbial growth on solid substrates under high-pressure conditions
3Productivity
If spray-type solid isolation is used, then the isolation efficiency is improved, but the manufacturing precision requirements increase
Solution Approach 1:
The patent changes the physical state of the culture medium from liquid to spray-applied liquid form. By controlling the spray parameters (pressure, nozzle size, droplet size distribution), the system achieves efficient isolation of marine microorganisms on solid supports without requiring extremely tight manufacturing tolerances, as the spray process naturally distributes the medium uniformly
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Improves the cultivability of marine microorganisms by enabling their enrichment and isolation in a dispersed state, achieving pure cultivation and providing a means for the development and utilization of high-pressure environment microbial resources.
Implementation Method 1
a pressurization system, a temperature environment control system... for constructing a high-pressure, low-temperature environment consistent with the marine environment
Implementation Method 2
The spray-type isolation and cultivation solid unit is use to convert the biological enrichment fluid into a state of microbeads, so that the biological enrichment fluid can be isolated and cultivated in a dispersed state
Implementation Method 3
a temperature environment control system... for constructing a high-pressure, low-temperature environment consistent with the marine environment
Data Source
AI summary
The invention proposes a high-pressure environment biological enrichment and spray-type solid isolation and cultivation device, including a spray-type isolation and cultivation solid unit, and a biological enrichment unit. In the case of constructing a high-pressure, low-temperature environment consistent with the marine environment, the biological enrichment unit is used to realize enrichment and multi-stage purification process of marine microorganisms, obtain a biological enrichment fluid and inject the biological enrichment fluid into the spray-type isolation and cultivation solid unit; and the spray-type isolation and cultivation solid unit is use to convert the biological enrichment fluid into a state of microbeads, so that the biological enrichment fluid can be isolated and cultivated in a dispersed state.