Histamine Detection Kit with Boric Acid Error Suppression
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Solution Overview
Problem
Methods for measuring histamine using histamine dehydrogenase often experience error reactions that interfere with detection, particularly in low-concentration ranges.
Innovation Solution
Incorporating boric acid, boronic acid, or alkyl sulfate into the measurement process to reduce error reactions, utilizing a kit or sensor that includes histamine dehydrogenase, a mediator, and a coloring reagent, with specific buffers and concentrations to enhance detection accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If histamine dehydrogenase is used for measurement, then measurement convenience and accuracy are improved, but error reactions occur that interfere with detection particularly in low-concentration ranges
Solution Approach 1:
The patent introduces boric acid or boronic acid as an intermediary substance that specifically binds to error reaction products, preventing them from interfering with histamine detection. The boronic acid acts as a mediator between the error reaction products and the detection system, selectively removing the interfering substances while allowing the enzyme-histamine reaction to proceed unimpeded.
Solution Approach 2:
The patent modifies the chemical environment by adding boric acid/boronic acid and adjusting pH parameters to optimize the enzyme reaction conditions. This parameter change suppresses error reactions while maintaining the catalytic activity of histamine dehydrogenase, thereby improving measurement precision without sacrificing convenience.
2Reliability
If conventional measurement methods are used, then measurement process is simple, but error reactions reduce reliability particularly in low-concentration detection
Solution Approach 1:
Boronic acid serves as a mediator that enhances detection reliability by selectively binding to error reaction products. This intermediary substance allows the system to maintain simplicity while improving reliability, as it can be added directly to the existing enzyme-based measurement system without requiring complex additional apparatus.
Solution Approach 2:
The measurement system is enhanced by combining histamine dehydrogenase with boric acid/boronic acid to create a composite measurement system. This composite approach integrates multiple functions (enzyme catalysis plus error reaction suppression) into a single unified system that maintains operational simplicity while improving reliability.
3Productivity
If histamine dehydrogenase method is employed, then rapid and convenient measurement is achieved, but error color development interferes with low-concentration detection
Solution Approach 1:
The boronic acid acts as an intermediary that rapidly binds to error reaction products, preventing their interference with low-concentration histamine detection. This allows the measurement system to maintain its rapid productivity while simultaneously improving precision in low-concentration ranges through the mediating action of boronic acid.
Solution Approach 2:
Boric acid or boronic acid is added to the measurement system in advance to preemptively suppress error reactions before they can interfere with histamine detection. This preliminary action ensures that both rapid measurement and precise low-concentration detection are achieved without compromise.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The use of boric acid or boronic acid significantly reduces error reactions, enabling accurate detection of histamine, even at low concentrations, by minimizing interference and improving measurement precision.
Implementation Method 1
histamine dehydrogenase
Implementation Method 2
oxidation of histamine
Implementation Method 3
boric acid or a salt thereof, and/or a boronic acid... capable of reducing the error reaction
Implementation Method 4
mediator... capable of detecting the oxidation of histamine
Data Source
AI summary
An object of the present invention is to reduce error reaction that is not derived from enzyme reaction in a method for measuring histamine using histamine dehydrogenase. The present invention provides a kit for detecting histamine or an electrochemical sensor capable of detecting the oxidation of histamine, comprising (i) histamine dehydrogenase, and (ii-a) boric acid or a salt thereof, and/or a boronic acid or a salt thereof, and/or (ii-b) alkyl sulfate. The present invention also provides a method for detecting histamine using (i) histamine dehydrogenase, and (ii-a) boric acid or a salt thereof, and/or a boronic acid or a salt thereof, or (ii-b) sodium laurel sulfate, or using an electrochemical sensor capable of detecting the oxidation of histamine, comprising these components.