Non-Invasive Histamine Sampling for Full Food Inspection
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Solution Overview
Problem
Conventional histamine measurement methods require invasive sampling by mincing edible parts, limiting the number of specimens that can be examined and making full inspection impossible, which is labor-intensive and time-consuming.
Innovation Solution
A non-invasive sampling method for histamine measurement that allows for determining the presence or absence of histamine in a specimen without destroying it, using techniques such as swabbing from specific sites like the mouth, gill slit, or surface of fish or chicken meat, followed by histamine measurement using histamine dehydrogenase or immunochromatography.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional histamine measurement methods are used requiring mincing of edible parts, then histamine measurement can be performed, but the specimen is destroyed and cannot be sold as a commercial product
Solution Approach 1:
The invention segments the sampling process by taking only a small portion of the specimen (e.g., from gill slit, mouth, or surface) rather than requiring mincing of the entire edible part. This allows histamine measurement while preserving the majority of the specimen for commercial use.
Solution Approach 2:
The invention extracts only the necessary sample material from specific locations (gill slit, mouth, surface) of the specimen rather than destroying the entire specimen through mincing. This extraction approach enables measurement while maintaining the specimen's commercial value.
2Measurement precision
If conventional histamine measurement methods requiring mincing are used, then histamine can be measured, but labor intensity increases and examination time increases
Solution Approach 1:
The invention performs preliminary sampling at specific locations (gill slit, mouth, surface) before the measurement process. This preliminary action simplifies subsequent measurement steps and reduces overall labor intensity and examination time compared to conventional mincing methods.
Solution Approach 2:
By extracting sample material from easily accessible locations such as gill slits, mouth, or surface, the invention eliminates the labor-intensive mincing process. This extraction approach significantly improves productivity while maintaining measurement accuracy.
3Measurement precision
If conventional histamine measurement methods are used, then histamine content can be determined, but full inspection of all specimens becomes impossible
Solution Approach 1:
The invention segments the inspection process into rapid, non-invasive sampling at key locations (gill slit, mouth, surface) that can be performed on multiple specimens efficiently. This segmentation enables full inspection of all specimens without the time-consuming mincing required by conventional methods.
Solution Approach 2:
By extracting samples from easily accessible locations rather than requiring destructive mincing, the invention makes it feasible to inspect all specimens thoroughly. This approach increases adaptability and versatility by allowing comprehensive screening while maintaining specimen integrity for commercial purposes.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and accurate histamine measurement without damaging the specimen, allowing for full inspection of all samples and preventing histamine food poisoning.
Implementation Method 1
histamine measurement by colorimetry using histamine dehydrogenase
Implementation Method 2
histamine dehydrogenase in the past
Implementation Method 3
antigen-antibody reaction
Implementation Method 4
non-invasively sampling a specimen to obtain a sample for measurement
Data Source
AI summary
An object of the present invention is to provide a convenient sampling method and kit for histamine measurement. The present invention provides a method and a kit which involve non-invasively sampling a specimen, and detecting histamine from the obtained sample.