HIV NC Protein Expression Vector with Intron and Stability Element

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Solution Overview

Problem

The expression of the HIV nucleocapsid (NC) protein in animal cells is challenging due to differences in viral codon usage and the presence of inhibitory sequences, leading to low expression efficiency, even with codon optimization alone.

Innovation Solution

A vector is developed that links an intron sequence and an mRNA stability element downstream of the NC gene, enhancing expression efficiency by optimizing codon usage and incorporating RNA stability elements.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If codon optimization is applied to HIV NC gene for expression in animal cells, then codon compatibility is improved, but expression efficiency remains low due to inhibitory sequences and lack of RNA stability elements

Engineering Contradiction:

Improvecodon compatibilityVSAvoidexpression efficiency

Core Design Contradiction:

Adaptability or versatilityVSProductivity

Solution Approach 1:

The patent combines multiple elements (codon-optimized NC gene, intron sequence, and mRNA stability element) into a single expression vector construct. This merging of components works synergistically to achieve high expression efficiency in animal cells, overcoming the limitations of codon optimization alone.

Inventive Principle:

Principle #5Merging (Combining)

Solution Approach 2:

The expression vector employs a composite genetic construct comprising codon-optimized NC gene sequence, intron sequence, and mRNA stability element. This composite structure integrates multiple functional elements that collectively enhance protein expression, analogous to using composite materials with combined properties.

Inventive Principle:

Principle #40Composite materials

2Productivity

If intron sequence and mRNA stability element are added to the expression vector, then expression efficiency is significantly improved, but vector complexity increases

Engineering Contradiction:

Improveexpression efficiencyVSAvoidvector structure

Core Design Contradiction:

ProductivityVSDevice complexity

Solution Approach 1:

The expression vector is segmented into distinct functional modules: the codon-optimized NC gene coding sequence, the intron sequence, and the mRNA stability element. This segmentation allows each component to perform its specific function while maintaining overall system efficiency and facilitating modular construction.

Inventive Principle:

Principle #1Segmentation

Solution Approach 2:

The expression vector design incorporates universal elements (intron sequence and mRNA stability element) that can be combined with different codon-optimized gene sequences to create versatile expression systems. These universal components provide multi-functional benefits across different application contexts.

Inventive Principle:

Principle #6Universality (Multi-functionality)

Data Source

PatentUS9738905B2Vector for expressing NC protein of HIV and method for producing NC protein using the same

Publication Date: 2017.08.22 AVIXGEN

AI summary

The present invention relates to a vector for expressing an NC protein of HIV and a method for producing an NC protein using the same. More particularly, the present invention relates to a vector for expressing an NC protein of HIV, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, and a method for producing an NC protein using the same. The vector for expressing an NC protein of HIV of the present invention, in which an intron sequence and an mRNA stability element in the downstream of NC gene are sequentially linked, can express a wild type NC protein in animal cells, and has an effect of improving the expression efficiency, as compared to a known art.