Hematopoietic Progenitor Cell Marker Selection for CD8+ T Cell Production
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Solution Overview
Problem
Current methods for inducing CD8 positive cells from hematopoietic progenitor cells have low efficiency and variation in yield, limiting their clinical application in cell therapy and diagnostics.
Innovation Solution
A method involving the separation of cells expressing specific HPC markers such as CD24, CD62L, CD90, CD143, and Notch3, and not expressing markers like CD49f, CD51, CD102, to differentiate into CD4/CD8 double positive cells, followed by induction into CD8 single positive cells using adrenocortical hormone agents and cytokines.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional methods are used to induce CD8 positive cells from hematopoietic progenitor cells, then cell production can proceed with existing protocols, but the induction efficiency is low and yield variation is high
Solution Approach 1:
The invention changes the parameters of cell separation by introducing specific HPC marker expressions (CD24+, CD62L+, CD90+, CD143+, Notch3+) and negative markers (CD49f-, CD51-, CD102-). This parameter-based selection approach transforms the induction process from low-efficiency conventional methods to a high-efficiency targeted approach, resolving the contradiction between productivity and reliability by establishing precise cellular criteria for optimal differentiation into CD8 positive cells
2Productivity
If cell separation using HPC markers is performed, then differentiation efficiency into CD8 positive cells increases, but the process complexity increases due to multiple marker detection
Solution Approach 1:
The invention applies preliminary action by performing cell separation using HPC markers before the differentiation induction step. By pre-enriching the hematopoietic progenitor cell population with the desired marker profile (CD24+, CD62L+, CD90+, CD143+, Notch3+, and negative for CD49f, CD51, CD102), the subsequent differentiation process becomes more efficient. This preliminary selection simplifies the overall process by ensuring only high-potency cells enter the differentiation stage, reducing waste and improving yield without requiring complex real-time monitoring during differentiation
Data Source
AI summary
Provided is a method for producing a CD4/CD8 double positive cell, including the following steps:step 1: separating, from a cell population containing a hematopoietic progenitor cell, a cell expressing one or more kinds of molecules selected from the first group consisting of CD24, CD62L, CD90, CD143, CD263, Notch3, CD32, CD39, CD49a, CD164, CD317, CD200, CD218a, CD7, CD144, CD56, CD226, CD262 and CD325, and/or a cell not expressing one or more kinds of molecules selected from the second group consisting of CD49f, CD51, CD102, CD42b, CD61, CD62P, CD69, CD102 and CD156c, andstep 2: differentiating the cell separated in step 1 into a CD4/CD8 double positive cell.