Hpp-5Mg Chromosome Enhances Homoeologous Recombination in Wheat
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for inducing homoeologous recombination in wheat breeding are inefficient, with low recombination frequencies and limited ability to introduce desirable traits from alien species, particularly due to the suppressive effect of the Ph1 gene, which restricts gene transfer to distal chromosome regions and results in low frequencies of successful recombination events.
Innovation Solution
The introduction of the Hpp-5Mg chromosome from Aegilops geniculata, which harbors promoter genes that enhance homoeologous pairing and crossover events, allowing for increased recombination frequencies and clustering of crossovers in proximal regions, thereby facilitating the transfer of alien genes into wheat.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the Ph1 gene is used to control homoeologous pairing, then pairing specificity is improved, but recombination frequency deteriorates
Solution Approach 1:
The patent extracts and removes the Ph1 gene from the wheat genome to eliminate its suppressive effect on homoeologous recombination. By deleting this specific gene, the invention enables frequent recombination events between homoeologous chromosomes while maintaining pairing specificity through alternative mechanisms, thus resolving the contradiction between pairing control and recombination frequency.
Solution Approach 2:
The patent changes the genetic parameter by introducing a translocation event that replaces the Ph1 gene's function with a new chromosomal arrangement. This parameter change allows homoeologous chromosomes to pair and recombine at high frequencies without the suppressive effect of Ph1, thereby improving recombination frequency while maintaining pairing specificity through the translocated chromosomal structure.
2Reliability
If Ph1 gene is present to prevent homoeologous pairing, then chromosome pairing control is improved, but gene transfer capability deteriorates
Solution Approach 1:
The invention extracts and removes the Ph1 gene from the wheat genome, eliminating its restrictive control on homoeologous pairing. This removal enables efficient gene transfer from alien species to wheat by allowing frequent recombination events, thus improving gene transfer capability while maintaining pairing control through the introduced translocation system.
Solution Approach 2:
The patent introduces a translocation event as an intermediary mechanism that mediates between pairing control and gene transfer. This translocation acts as a bridge, allowing homoeologous chromosomes to pair and recombine efficiently without the need for Ph1 gene removal, thus maintaining pairing control while enabling effective gene transfer from alien species.
3Stability of the object's composition
If recombination is restricted to distal regions, then pairing stability is improved, but accessibility of genetic variation deteriorates
Solution Approach 1:
The patent changes the chromosomal parameter by introducing a translocation that repositions genetic material and alters the chromosomal architecture. This parameter change enables recombination events to occur in proximal regions that were previously inaccessible, while maintaining pairing stability through the new chromosomal arrangement. Consequently, genetic variation becomes accessible across the entire chromosome, not just in distal regions.
Data Source
AI summary
Wheat lines comprising homoeologous pairing promoter genes from chromosome 5Mg from Ae. geniculata and methods of inducing homoeologous recombination in plant breeding from these lines.