HPV Genotype Detection Oligonucleotide Probe Sets
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Solution Overview
Problem
Current HPV detection methods are inadequate for simultaneously detecting multiple high-risk HPV genotypes while differentiating between them, and they often result in storage and reagent waste, requiring longer processing times.
Innovation Solution
A set of oligonucleotide sequences, including forward primers, reverse primers, and probes, specifically designed to amplify and detect HPV genotypes 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and 68, allowing for rapid detection in a single reaction, compatible with lyophilization for reduced storage needs and unit-dose format to minimize reagent waste.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple HPV genotypes are detected in a single reaction, then detection efficiency is improved, but differentiation precision between specific genotypes deteriorates
Solution Approach 1:
The patent divides the detection system into multiple probe sets, each specifically designed to hybridize with and detect a particular HPV genotype (16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, or 68). Each probe contains a unique sequence complementary to its target genotype, allowing simultaneous detection while maintaining clear differentiation through genotype-specific signal patterns.
Solution Approach 2:
Each probe in the mixture has locally optimized sequence characteristics tailored to its specific target genotype. The probes differ in their nucleotide sequences, binding affinities, and hybridization conditions, enabling each to selectively bind to its corresponding genotype while allowing all probes to coexist in the same reaction mixture without cross-interference.
2Reliability
If conventional HPV detection methods are used, then detection capability is provided, but storage requirements and reagent waste increase
Solution Approach 1:
The patent combines multiple genotype-specific probes and amplification reagents into a single integrated reaction mixture. This unified approach allows all necessary components for detecting 14 different HPV genotypes to be present in one test, eliminating the need for separate reactions for each genotype and thereby reducing reagent consumption and waste.
Solution Approach 2:
The reaction system is designed with universal components that can detect multiple HPV genotypes simultaneously. The primer set and probe mixture are formulated to work together in a single PCR reaction, providing multi-functional detection capability that replaces multiple separate assays and reduces overall reagent requirements.
3Reliability
If conventional HPV detection methods are used, then detection is performed, but processing time increases
Solution Approach 1:
The patent employs a pre-optimized probe mixture where all necessary genotype-specific probes are prepared and combined in advance at appropriate concentrations. The primer and probe sequences are pre-designed to ensure efficient amplification and hybridization, allowing the detection reaction to proceed rapidly without requiring sequential processing or additional preparation steps during the assay.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables rapid and efficient detection of multiple high-risk HPV genotypes with reduced storage and reagent requirements, facilitating quicker turnaround times and improved diagnostic capabilities.
Implementation Method 1
a first probe oligonucleotide sequence comprising SEQ ID NO: 7 or SEQ ID NO: 25 which specifically hybridizes to HPV genotype 16
Data Source
AI summary
The disclosure is directed to kits and methods for amplifying and detecting human papilloma virus (HPV) of genotype 16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66, and/or 68 in a sample, which comprises a variety of combinations of forward oligonucleotide primers, reverse oligonucleotide primers, and oligonucleotide probes.