HPV Genotype Detection Kit Using Degenerate PCR Primers
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Solution Overview
Problem
Current HPV detection methods lack comprehensive detection of medium and high-risk genotypes, leading to suboptimal detection rates and potential miss-detection, which is crucial for early diagnosis and quality control of cervical cancer.
Innovation Solution
A method and kit for detecting 65 HPV genotypes, including 13 high-risk and 5 medium-risk genotypes, using phylogenetic analysis to identify closely related HPV strains and employing PCR assays with degenerate primer pairs to enhance detection efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional HPV detection methods are used, then detection of common HPV genotypes is achieved, but detection of medium and high-risk genotypes is incomplete leading to miss-detection
Solution Approach 1:
The patent applies universality by designing a detection system that can identify multiple HPV genotypes (including 13 high-risk and 5 medium-risk genotypes) using a unified phylogenetic analysis approach. The method uses universal primer pairs that can bind to conserved regions across diverse HPV genotypes, enabling a single test to screen for numerous genotype variants simultaneously, thus achieving comprehensive detection without requiring separate tests for each genotype.
Solution Approach 2:
The patent employs parameter changes by utilizing phylogenetic analysis based on DNA sequence variations in the L1 region. By analyzing sequence divergence and establishing phylogenetic trees, the method dynamically adjusts detection parameters to distinguish between different genotype groups. This allows the system to adapt to genetic variations and maintain high detection accuracy across diverse HPV strains.
2Reliability
If phylogenetic analysis is used to identify HPV genotypes, then comprehensive genotype detection is achieved, but detection complexity increases
Solution Approach 1:
The patent applies segmentation by dividing the complex task of HPV genotype detection into manageable steps: DNA extraction, PCR amplification of the L1 region, sequence analysis, and phylogenetic classification. By segmenting the detection process, the system makes complex phylogenetic analysis more tractable and implementable in clinical settings while maintaining comprehensive detection capabilities.
Solution Approach 2:
The patent uses phylogenetic trees as an intermediary tool to simplify genotype identification. Instead of directly comparing all possible genotype sequences, the method constructs phylogenetic trees that serve as a reference framework, allowing unknown samples to be classified by comparing their sequences to the established tree structure. This intermediary approach reduces computational complexity while maintaining accurate genotype identification.
3Reliability
If multiple HPV genotypes are detected simultaneously, then miss-detection is reduced, but detection time and resource requirements increase
Solution Approach 1:
The patent merges multiple detection functions into a single unified assay. By using universal primer pairs that can amplify conserved regions across all HPV genotypes and applying phylogenetic analysis to the amplified product, the system combines what would traditionally require multiple separate tests into one comprehensive detection procedure, thereby reducing overall detection time while maintaining completeness.
Solution Approach 2:
The patent employs preliminary action by pre-establishing phylogenetic trees and classification criteria before actual sample detection. The reference phylogenetic framework is constructed in advance based on known HPV genotype sequences, allowing rapid classification of new samples through simple sequence comparison rather than requiring de novo analysis for each sample, thus reducing detection time.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method and kit provide maximized detection of HPV genotypes, reducing miss-detection and improving disease screening efficiency, ensuring product quality and safety for biological products.
Implementation Method 1
the presence of said genotypes is detected by polymerase chain reaction (PCR) assays
Data Source
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AI summary
Provided is a method and a kit for detection of human papillomaviruses. Specifically, provided is a method for detecting at least 65 human papillomavirus genotypes. Also provided is a kit for detection of human papillomaviruses, which comprises one or more reagents capable of detecting at least 65 human papillomavirus genotypes.