HRH4 mRNA Detection Reagent for Allergic Disease Monitoring
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Solution Overview
Problem
Current treatments for allergic diseases, particularly those targeting the H1 receptor, are ineffective for all patients and lack quantifiable therapeutic effects, leading to side effects from long-term medication, with no reliable method to detect the expression level of the human histamine receptor HRH4 mRNA.
Innovation Solution
A reagent and kit for detecting the expression level of human histamine receptor HRH4 mRNA using specific primers and probes, enabling one-step quantitative real-time PCR without separate reverse transcription, with fluorescent labeling for high sensitivity and accuracy, allowing for the detection of HRH4 mRNA in various samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If H1 receptor antagonists are used for treating allergic diseases, then some allergic symptoms can be relieved, but the treatment is not effective for all patients and lacks quantifiable therapeutic effects
Solution Approach 1:
The patent introduces a detection method that provides feedback on HRH4 mRNA expression levels in patients. This enables clinicians to quantify the therapeutic effect of H4 receptor antagonists by monitoring changes in HRH4 expression before and after treatment, allowing for personalized treatment adjustments and objective evaluation of therapeutic response.
Solution Approach 2:
The patent shifts the detection parameter from traditional H1 receptor activity to HRH4 mRNA expression levels. By detecting and quantifying HRH4 mRNA expression, the method provides a measurable parameter that reflects the underlying mechanism of action and therapeutic response, enabling objective assessment of treatment effectiveness across different patient populations.
2Duration of action of stationary object
If long-term medication is administered to treat allergic diseases, then therapeutic effects are maintained, but side effects are produced
Solution Approach 1:
The detection method provides feedback on HRH4 mRNA expression levels, enabling monitoring of therapeutic response over time. This allows clinicians to adjust medication duration and dosage based on actual treatment response, potentially reducing the need for long-term medication and minimizing associated side effects while maintaining therapeutic effectiveness.
Solution Approach 2:
The patent replaces subjective clinical assessment with objective molecular detection of HRH4 mRNA expression. This substitution enables precise quantification of therapeutic effect, allowing for more precise treatment duration optimization and reducing unnecessary long-term medication that causes side effects.
3Measurement precision
If traditional detection methods are used for HRH4 expression, then detection can be performed, but accuracy and sensitivity are insufficient
Solution Approach 1:
The patent uses quantitative real-time PCR technology to create and detect copies of HRH4 mRNA through reverse transcription and amplification. This copying process enables sensitive detection of low-abundance mRNA molecules, significantly improving detection sensitivity and accuracy compared to traditional methods.
Solution Approach 2:
The patent changes the detection parameter from direct protein detection to mRNA expression level quantification. By detecting HRH4 mRNA expression levels, the method provides a more sensitive and accurate measure of receptor presence and therapeutic response, overcoming the limitations of traditional detection methods.
4Measurement precision
If multi-step detection procedures are used, then comprehensive analysis can be performed, but detection time is extended
Solution Approach 1:
The patent merges reverse transcription and quantitative PCR into a single one-step procedure. This combination eliminates the need for separate reverse transcription and amplification steps, significantly reducing detection time while maintaining comprehensive analysis capability through real-time fluorescence monitoring of HRH4 mRNA expression.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method provides high accuracy and sensitivity for detecting HRH4 mRNA levels in human samples, enabling precise monitoring of therapeutic effects and guiding medication dosage, reducing aerosol pollution and detection time, and spanning a wide range of concentrations.
Implementation Method 1
the probe includes an H4-Probe; and the H4-Probe has a nucleotide sequence shown in SEQ ID NO. 3
Data Source
AI summary
The present disclosure provides a reagent for detecting an expression level of a human histamine receptor HRH4 mRNA, a kit and a detection method. In the present disclosure, the reagent includes a specific primer and a probe for a human histamine receptor HRH4, the specific primer includes an HRH4-F and an HRH4-R, and the probe includes an H4-Probe; and the HRH4-F has a nucleotide sequence shown in SEQ ID NO. 1, the HRH4-R has a nucleotide sequence shown in SEQ ID NO. 2 and the H4-Probe has a nucleotide sequence shown in SEQ ID NO. 3. In the present disclosure, a kit for one-step detection and a detection method based on the reagent are prepared, and the expression level of the HRH4 mRNA can be one-step quantitatively detected with simple operation and short detection time.