High-Speed Countercurrent Chromatography for Cannabinoid Separation
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Solution Overview
Problem
There is no existing technology for the simultaneous separation of cannabidivarin and cannabigerol using high-speed countercurrent chromatography, which is necessary for achieving high purity levels of these compounds for neurological disorder treatments.
Innovation Solution
A method employing high-speed countercurrent chromatography with a solvent system comprising n-hexane or n-heptane, methyl tert-butyl ether, acetonitrile, or ethanol, and water in specific volume ratios, allowing for the separation and purification of cannabidivarin and cannabigerol with purities greater than 98% and 97%, respectively, by using the upper phase as the stationary phase and the lower phase as the mobile phase, and optimizing conditions such as rotation speed, temperature, and flow rate.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Manufacturing precision
If conventional extraction methods are used to obtain cannabidivarin and cannabigerol from industrial hemp, then the compounds can be extracted, but simultaneous separation and purification to high purity levels cannot be achieved
Solution Approach 1:
The patent changes the parameters of the solvent system (using n-hexane or n-heptane as stationary phase and ethanol or methanol as mobile phase) and optimization conditions (rotation speed, temperature, flow rate) to achieve simultaneous separation of cannabidivarin and cannabigerol at high purity levels greater than 98% and 97% respectively
Solution Approach 2:
The patent replaces conventional mechanical extraction methods with high-speed countercurrent chromatography, which uses liquid-liquid partitioning principles to achieve separation without requiring solid support or carrier, thereby simplifying the process while achieving high purity
2Productivity
If high-speed countercurrent chromatography is used for separation, then separation efficiency is improved, but no existing method achieves simultaneous separation of cannabidivarin and cannabigerol
Solution Approach 1:
The patent segments the cannabinoid mixture into separate phases using liquid-liquid partitioning, where cannabidivarin and cannabigerol are distributed between the stationary phase (n-hexane or n-heptane) and mobile phase (ethanol or methanol) based on their different solubility characteristics, enabling simultaneous separation
Solution Approach 2:
The patent optimizes parameters including rotation speed (800 rpm), column temperature (25°C), and flow rate (5 mL/min) to achieve efficient simultaneous separation of both compounds in a single run, demonstrating high productivity and reliability
3Manufacturing precision
If traditional purification methods are applied, then some purification can be achieved, but sample loss and contamination occur
Solution Approach 1:
The patent replaces traditional purification methods with high-speed countercurrent chromatography that uses liquid-liquid partitioning without solid support or carrier, eliminating irreversible adsorption and preventing sample loss while achieving high purity levels greater than 98% for cannabidivarin and 97% for cannabigerol
Solution Approach 2:
The patent enables recovery and recycling of the solvent system (n-hexane or n-heptane, ethanol or methanol) after separation, reducing waste and maintaining high purity levels without sample loss, as the solvents can be reused in subsequent separation cycles
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method achieves high-purity simultaneous separation and purification of cannabidivarin and cannabigerol with advantages including no sample loss, high efficiency, and environmentally friendly solvent recycling, while ensuring high production volumes and minimal contamination.
Implementation Method 1
High-speed countercurrent chromatography (HSCCC) is a new separation and purification technique based on the principle of liquid-liquid partitioning
Implementation Method 2
the solvent system is obtained by mixing n-hexane or n-heptane, methyl tert-butyl ether, acetonitrile or ethanol, and water in a volume ratio of 5~10: 1∼3: 5∼10: 2∼4
Data Source
AI summary
The present invention relates to a method for simultaneously separating cannabidivarin and cannabigerol, comprising: sufficiently oscillating a solvent system, allowing the solvent system to stand, and separately collecting an upper phase and a lower phase; dissolving a commercially available industrial hemp full-spectrum refined oil into the upper phase, performing separation using high-speed countercurrent chromatography with the upper phase as a stationary phase and the lower phase as a mobile phase to obtain a mixture of cannabidivarin and the mobile phase and a mixture of cannabigerol and the mobile phase, respectively, and removing the mobile phase to obtain cannabidivarin and cannabigerol. The present invention uses high-speed countercurrent chromatography to simultaneously separate and purify to obtain cannabidivarin (CBDV) with the purity of greater than 98% and cannabigerol (CBG) with the purity of greater than 97% from the industrial hemp full-spectrum refined oil for the first time.