Human-Derived Insecticidal Peptide Binds Cry2Aa Toxin
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Solution Overview
Problem
The widespread use of transgenic Bt toxin crops poses potential safety hazards, including gene escape, microbial ecological changes, drug resistance, and immunosuppression, necessitating the development of alternative biological control agents with similar bioactivity.
Innovation Solution
A human-derived insecticidal gene encoding a β-type anti-Cry2Aa toxin single-chain antibody (insecticidal peptide) is identified and expressed using a prokaryotic system, demonstrating binding activity to specific insect receptors without animal immunization, suitable for in vitro mass production.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic Bt toxin crops are widely used for biological control, then pest control effectiveness is improved, but potential safety hazards including gene escape, microbial ecological changes, drug resistance, and immunosuppression occur
Solution Approach 1:
The patent creates a human-derived single-chain antibody that copies the binding function of the native receptor to recognize and bind Cry2Aa toxin, providing an alternative mechanism for pest control that avoids the safety issues associated with transgenic Bt crops while maintaining effectiveness against target pests
Solution Approach 2:
The single-chain antibody acts as an intermediary substance that mediates the interaction between the toxin and the receptor, blocking the toxin's harmful effects without requiring transgenic modification of crops, thereby reducing safety hazards while maintaining pest control effectiveness
2Productivity
If Bt toxin protein is used at large doses for pest control, then insecticidal effect is improved, but immunosuppression and toxicity to normal immune system occur
Solution Approach 1:
The patent develops a single-chain antibody that replicates the receptor's binding capability to Cry2Aa toxin, providing an alternative approach to achieve insecticidal effect without using large doses of Bt toxin protein that cause immunosuppression
Solution Approach 2:
The patent converts the harmful immunosuppressive effect of Bt toxin into a beneficial outcome by developing a single-chain antibody that achieves pest control without the toxic side effects, transforming the problem of toxin toxicity into a solution using a safe alternative mechanism
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The insecticidal peptide effectively controls agricultural pests, offering a safer alternative to Bt toxins by reducing long-term toxicity risks and maintaining bioactivity, thus providing a new resource for biological pest control.
Implementation Method 1
the primary culture of the insecticidal peptide has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV
Data Source
AI summary
The present invention discloses a human-derived insecticidal gene and insecticidal peptide encoded by the same and application thereof. The nucleotide sequence of the human-derived insecticidal gene is as represented by SEQ ID NO.1. The amino acid sequence of the insecticidal peptide encoded by this gene is as represented by SEQ ID NO.2. The insecticidal peptide may be expressed through prokaryotic system. The primary culture has binding activity to Cnaphalocrocis medinalis midgut peritrophic membrane specific receptor BBMV. It is obtained without animal immunization and has a short production cycle and a small amino acid sequence. It is suitable for in vitro mass production and may lower the safety risks resulting from wide use of existing Bt toxins and even might substitute Bt to biologically control agricultural pests in the future. It has important scientific and practical significance to reducing the use of insecticides.