Human Serum for Stem Cell Culture via Glass-Activated Platelet Release
Solution Overview
Problem
Current methods for preparing human serum for stem cell culture are inefficient, as they require repeated procedures, pose infection risks, or use plasma with insufficient cell growth factors, making them unsuitable for practical applications in regenerative medicine.
Innovation Solution
A human serum for cell culture is produced using whole blood with a residual platelet ratio of 0-20% and a release ratio of cell growth factors of 20-100%, utilizing a serum preparation apparatus with a processed glass body to activate platelets and coagulation factors, resulting in a serum with a higher content of growth factors comparable to fetal bovine serum.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If a low capacity blood collection tube is used for serum preparation, then the serum can be obtained through a simple procedure, but the preparatory procedures must be repeated many times to prepare sufficient serum for stem cell culture
Solution Approach 1:
The invention divides the blood collection process into multiple separate collections from the same subject, where each collection is processed individually through simple procedures, and the serums are combined to achieve the required total amount for stem cell culture
Solution Approach 2:
The invention performs preliminary blood collections from the same subject before the actual stem cell culture is needed, allowing multiple simple processing procedures to be completed in advance and the serums to be stored for later use, thereby avoiding the need to repeat procedures at the time of culture
2Ease of manufacture
If thrombin is used as an anticoagulant to produce human plasma, then the plasma can be obtained, but there is an infection risk due to the biologic origin of thrombin
Solution Approach 1:
The invention extracts and removes thrombin and other coagulation factors from the blood during the serum preparation process, using only the serums from multiple blood collections without adding any biologic substances, thereby eliminating the infection risk associated with biologic anticoagulants while still achieving the goal of producing human plasma for culture
Solution Approach 2:
The invention uses disposable, non-biologic materials and methods for blood collection and processing, replacing the need for biologic anticoagulants like thrombin with simple mechanical or chemical methods that carry no infection risk
3Ease of manufacture
If plasma with small amount of cell growth factors is used as raw material, then the serum production is simplified, but the resulting serum does not contain sufficient cell growth factors for efficient stem cell culture
Solution Approach 1:
The invention combines serums from multiple blood collections from the same subject, where each serum individually has a moderate amount of growth factors, but when combined they achieve the sufficient concentration and total amount of cell growth factors needed for efficient stem cell culture while maintaining simple production procedures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The serum efficiently promotes stem cell proliferation, providing a safer and more effective medium for regenerative medicine by increasing the amount of cell growth factors, reducing contamination risks, and ensuring high safety and scalability.
Implementation Method 1
a blood coagulation accelerating solid, such as glass powder
Implementation Method 2
utilizing a serum preparation apparatus with a processed glass body to activate platelets and coagulation factors
Data Source
AI summary
It is intended to provide a serum which contains a large amount of growth factors capable of efficiently promoting the growth of stem cells. A human serum for cell culture which shows a residual ratio of platelets remaining within 20 minutes after blood collection in relation to the whole amount of the platelets is 0% to 20%, and a release ratio of cell growth factors is 20% to 100%.