Single-Channel Hybrid Biosensor for Multi-Biomarker Detection
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Solution Overview
Problem
Conventional immuno-biosensors for point-of-care testing (POCT) are inadequate for simultaneously measuring multiple biomarkers through chemical and binding reactions, leading to complex assay systems and reduced diagnostic reliability, especially for diseases like diabetes that require information on both types of markers.
Innovation Solution
A single-channel, chemical-immunological hybrid biosensor that integrates immuno-reaction zones and chemical reaction zones on a porous membrane strip, allowing for simultaneous and independent measurement of heterologous multiple biomarkers using capillary action, with signal-generating substrates like Triton X-100, bromocresol green, and luminol to generate chromogenic and luminescent signals for diabetes diagnosis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If conventional immuno-biosensors are used for POCT, then high sensitivity for single biomarker detection is achieved, but the ability to simultaneously measure chemical reaction markers is lost
Solution Approach 1:
The patent merges chemical reaction zones and immuno-reaction zones into a single integrated biosensor device. The reaction strip contains both types of reaction zones that can simultaneously perform chemical assays (e.g., hemoglobin measurement) and immunoassays (e.g., HbA1c measurement), allowing one device to replace multiple separate devices while maintaining the sensitivity of immunoassay detection.
2Adaptability or versatility
If separate channels are used to measure heterologous multiple biomarkers, then different assay types can be performed, but device complexity and diagnostic reliability deteriorate
Solution Approach 1:
The reaction strip is segmented into distinct functional zones including a chemical reaction zone and one or more immuno-reaction zones. Each zone is spatially separated and optimized for its specific assay type, yet all zones operate within a single integrated device. This segmentation allows multiple biomarkers to be measured simultaneously without requiring complex multi-device systems.
3Quantity of substance
If multiple blood samples are collected frequently, then sufficient sample volume for multiple assays can be obtained, but patient discomfort and sample wastage increase
Solution Approach 1:
The biosensor device is designed with universal functionality to perform multiple different assays (chemical and immunoassays) simultaneously using a single blood sample. The reaction strip can measure various biomarkers including hemoglobin, HbA1c, and other analytes in parallel, making the device adaptable to different diagnostic needs without requiring additional samples or devices.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables accurate and rapid diagnosis of diseases like diabetes by simultaneously analyzing multiple biomarkers in a single sample, reducing patient discomfort and sample wastage, and facilitating on-site, economical, and convenient analysis.
Implementation Method 1
a reaction strip in the form of a porous membrane through which a sample moves by capillary action
Implementation Method 2
at least one chemical reaction zone including a matrix to supply substrate(s) chemically reacting with a chemical reaction marker in the sample to generate a second reaction signal
Implementation Method 3
one or more immuno-reaction zones including capture recognition elements specifically binding to binding reaction markers in the sample
Implementation Method 4
signal-generating substrates like Triton X-100, bromocresol green, and luminol to generate chromogenic and luminescent signals
Implementation Method 5
luminol to generate chromogenic and luminescent signals
Data Source
AI summary
A single-channel, chemical-immunological hybrid biosensor is disclosed. According to an embodiment, the hybrid biosensor includes a reaction strip 100 in the form of a porous membrane through which a sample 1 moves by capillary action. the reaction strip 100 is in the form of a porous membrane through which the sample 1 moves by capillary action. Biomarkers in the sample 1 are independently detected based on a chemical reaction and binding reactions on the reaction strip 100.