Hybrid Corn CH010392 CMS Breeding and Tissue Culture
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Solution Overview
Problem
The challenge in corn breeding is to develop hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination.
Innovation Solution
The development of the hybrid corn variety CH010392, which includes genetic modifications and cytoplasmically-inherited traits to confer characteristics like male sterility, herbicide resistance, and disease resistance, while using tissue culture techniques to regenerate plants with consistent physiological and morphological characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If self-pollination is prevented through male sterility mechanisms, then cross-pollination and hybrid vigor are improved, but self-pollination control becomes more complex
Solution Approach 1:
The patent extracts the male fertility function by introducing cytoplasmic male sterility (CMS) into the female parent line, effectively removing the ability of the female plant to self-pollinate. This extraction of the male reproductive function from the female parent ensures complete dependence on cross-pollination for seed production, resolving the contradiction by making cross-pollination control reliable without adding mechanical complexity.
Solution Approach 2:
The patent uses a restorer line as an intermediary to control pollination. The restorer line contains nuclear restorer genes that can restore male fertility when crossed with CMS lines. By using this genetic intermediary, the system achieves reliable cross-pollination control through biological mechanisms rather than mechanical or chemical interventions, simplifying the overall control mechanism.
2Productivity
If multiple desirable traits are combined in a single hybrid variety, then overall performance and productivity are improved, but genetic stability becomes more difficult to maintain
Solution Approach 1:
The patent segments the hybrid breeding process into distinct parental lines, each contributing specific traits. The female parent (CV399111) contributes yield potential and stress tolerance, while the male parent (CV399175) contributes disease resistance and uniformity. By segmenting the trait contribution across separate homozygous inbred lines, the system maintains genetic stability within each parent line while achieving superior overall performance in the hybrid through controlled cross-pollination.
3Productivity
If tissue culture techniques are used to regenerate plants, then propagation speed and uniformity are improved, but genetic variability may be reduced
Solution Approach 1:
The patent uses tissue culture techniques to create identical copies of selected hybrid plants. By regenerating plants from callus or other tissue cultures, the system produces genetically uniform propagules that maintain the desired hybrid characteristics. This copying approach accelerates propagation while preserving the genetic integrity of the selected hybrid, as the tissue culture process maintains the heterozygous genotype without the segregation that occurs in sexual reproduction.
Data Source
AI summary
According to the invention, there is provided seed and plants of the hybrid corn variety designated CH010392. The invention thus relates to the plants, seeds and tissue cultures of the variety CH010392, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010392 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH010392.