Hybrid Corn CH010977 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and preventing self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH010977, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with tissue culture methods for regenerating plants with specific physiological and morphological characteristics, and the use of genetic editing techniques like CRISPR-Cas systems for introducing desired traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If conventional breeding techniques are used to develop hybrid corn varieties, then genetic diversity and trait combination are improved, but self-pollination occurs which reduces hybrid seed production efficiency

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidself-pollination prevention
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent extracts and removes the male reproductive capability from the female parent line by introducing cytoplasmic male sterility (CMS). This eliminates pollen production in the female parent, preventing self-pollination and ensuring that only cross-pollinated seeds are produced, thereby improving hybrid seed production efficiency while maintaining reliable trait combination.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses a male sterility system as an intermediary mechanism to control pollination. By introducing CMS cytoplasm and corresponding nuclear restorer genes, the system mediates between the desire for genetic diversity (through cross-pollination) and the need to prevent self-pollination, enabling efficient hybrid seed production with reliable trait inheritance.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple desirable traits are combined in a single hybrid variety, then agronomic performance is improved, but genetic stability becomes difficult to maintain

Engineering Contradiction:
Improveagronomic performanceVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent segments the genetic material into distinct parental lines, each contributing specific desirable traits. By dividing the breeding program into separate inbred lines with defined trait combinations, the patent maintains genetic stability within each line while achieving improved agronomic performance through their hybrid combination, preventing trait degradation over generations.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent changes the genetic parameters of parental lines through repeated self-pollination and selection to achieve homozygosity and stability. By adjusting the genetic composition parameters of parent lines before crossing, the patent ensures that the resulting hybrid maintains stable inheritance of multiple desirable traits while achieving superior agronomic performance.

Inventive Principle:
Principle #35Parameter changes

3Manufacturing precision

If inbred plants are developed through repeated self-pollination to achieve uniformity, then phenotypic consistency is improved, but inbreeding depression reduces yield potential

Engineering Contradiction:
Improvephenotypic uniformityVSAvoidyield potential
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The patent merges two different inbred lines with complementary genetic backgrounds to produce the hybrid. This combination restores heterosis (hybrid vigor), achieving both phenotypic uniformity (through controlled crossing) and high yield potential (through heterotic effect), overcoming the limitations of inbreeding depression while maintaining the desired uniformity in the hybrid population.

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS11980151B2Plants and seeds of hybrid corn variety CH010977
Publication Date: 2024.05.14 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH010977. The invention thus relates to the plants, seeds and tissue cultures of the variety CH010977, and to methods for producing a corn plant produced by crossing a corn plant of variety CH010977 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH010977.