Hybrid Corn CH011022 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the introduction of new genetic modifications and cytoplasmically inherited traits.

Innovation Solution

The development of the hybrid corn variety CH011022, which incorporates genetic modifications for traits like herbicide resistance, male sterility, and disease resistance, utilizing cytoplasmic male sterility (CMS) and restorer genes, along with advanced breeding methods like backcrossing and genetic transformation techniques to introduce desired loci, ensuring stable expression of traits across generations.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional self-pollination breeding methods are used to develop uniform corn hybrids, then genetic stability and uniformity are achieved, but the ability to introduce new genetic modifications and cytoplasmically inherited traits is limited

Engineering Contradiction:
Improveability to introduce new genetic traitsVSAvoidgenetic stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The invention segments the genetic material into two distinct components: nuclear DNA and mitochondrial DNA (cytoplasm). By utilizing cytoplasmic male sterility (CMS), the patent enables independent inheritance and manipulation of mitochondrial traits separate from nuclear traits. This segmentation allows simultaneous introduction of cytoplasmically inherited traits (like male sterility) and nuclear genetic modifications while maintaining overall genetic stability through controlled breeding systems.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs restorer genes as intermediaries that can counteract the effects of cytoplasmic male sterility. These restorer genes act as mediators between the CMS cytoplasm and normal fertility, allowing controlled introduction of cytoplasmic traits while maintaining breeding flexibility. The restorer genes enable the system to switch between sterile and fertile states, facilitating the introduction of new genetic modifications without compromising genetic stability.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Manufacturing precision

If cytoplasmic male sterility (CMS) and restorer genes are used to prevent self-pollination and enable hybrid breeding, then uniformity and hybrid vigor are improved, but breeding program complexity increases

Engineering Contradiction:
Improveuniformity in germination and growthVSAvoidbreeding program complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent utilizes self-service mechanisms where the CMS trait automatically prevents self-pollination without requiring manual intervention. The cytoplasmic male sterility inherently blocks pollen production in female parent plants, eliminating the need for manual emasculation or other labor-intensive self-pollination prevention techniques. This self-service approach maintains uniformity while reducing operational complexity in hybrid seed production.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent implements preliminary action by pre-establishing CMS lines and restorer lines before hybrid production. These parental lines are developed and characterized in advance, with CMS lines already possessing the male-sterile trait and restorer lines containing the fertility-restoring genes. This preliminary preparation simplifies the actual hybridization process, as breeders only need to cross pre-characterized lines rather than managing complex breeding programs during production.

Inventive Principle:
Principle #10Preliminary action

3Adaptability or versatility

If advanced breeding methods like backcrossing and genetic transformation are employed to introduce desired loci, then trait diversity and adaptability increase, but time and resource investment increase

Engineering Contradiction:
Improvetrait diversityVSAvoidbreeding development time
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (repeated backcrossing and phenotypic selection) with genetic transformation techniques. Instead of relying on time-consuming mechanical crossing and selection processes, the invention uses molecular biology methods to directly introduce desired genes into the genome. This substitution dramatically reduces breeding development time while maintaining trait diversity, as specific loci can be precisely inserted without requiring multiple generations of backcrossing.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent utilizes parameter changes by modifying specific genetic parameters (presence/absence of desired loci) through targeted genetic transformation. Rather than changing the entire genetic background through extensive backcrossing, the invention changes only the specific parameters needed (introducing or modifying particular genes) while maintaining the rest of the genetic structure. This approach reduces time investment by focusing modifications on specific loci rather than whole-genome reshuffling.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11317581B2Plants and seeds of hybrid corn variety CH011022
Publication Date: 2022.05.03 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH011022. The invention thus relates to the plants, seeds and tissue cultures of the variety CH011022, and to methods for producing a corn plant produced by crossing a corn plant of variety CH011022 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH011022.