Hybrid Corn CH011430 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH011430, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with specific genetic loci conferring herbicide resistance, insect resistance, and other desirable characteristics, using advanced breeding methods and genetic transformation techniques like CRISPR-Cas systems.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional breeding methods are used to develop hybrid corn varieties, then genetic diversity and desirable traits can be combined, but self-pollination occurs which reduces hybrid seed production efficiency

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidself-pollination control
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent extracts and removes the male reproductive capability from the female parent line by inducing cytoplasmic male sterility (CMS). This is achieved by introducing specific mitochondrial DNA mutations that disrupt pollen development, effectively taking out the self-pollination capability from the system while preserving the female parent's other desirable traits.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The patent uses a restorer gene as an intermediary element that, when introduced into the hybrid, restores male fertility. This restorer gene acts as a mediator between the sterile cytoplasm and the nuclear genome, allowing controlled restoration of pollen production in the F1 hybrid while maintaining sterility in the female parent line.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple desirable traits are combined in a single hybrid variety, then yield and resistance improve, but genetic stability becomes difficult to maintain

Engineering Contradiction:
Improveyield and resistanceVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent segments the genetic material into distinct components: a female parent line with sterile cytoplasm containing certain nuclear genes, and a male parent line with fertile cytoplasm containing complementary nuclear genes. This segmentation allows each parent line to be independently optimized and maintained for genetic stability, while the hybrid combines all desirable traits from both parents.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies local quality by creating specific regional differences in the genetic composition of different parent lines. The female parent line is locally optimized for sterility and specific traits, while the male parent line is locally optimized for fertility and complementary traits, allowing each to maintain genetic stability in its specific context.

Inventive Principle:
Principle #3Local quality

3Productivity

If cytoplasmic male sterility is induced to prevent self-pollination, then hybrid seed production efficiency improves, but the complexity of breeding programs increases

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidbreeding program complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent implements self-service by designing a system where the female parent line automatically prevents self-pollination through cytoplasmic male sterility, eliminating the need for manual emasculation or other complex interventions. The sterile cytoplasm serves itself to enforce cross-pollination without requiring additional breeding program complexity.

Inventive Principle:
Principle #25Self-service

4Productivity

If genetic transformation techniques are used to introduce desirable traits, then trait incorporation efficiency improves, but the risk of genetic instability increases

Engineering Contradiction:
Improvetrait incorporation efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent merges multiple breeding approaches by combining traditional cross-breeding with cytoplasmic male sterility induction. This integration allows efficient incorporation of desirable traits through controlled crosses while the CMS system maintains genetic stability by preventing unintended self-pollination and ensuring true-to-type hybrid production.

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS11419289B2Plants and seeds of hybrid corn variety CH011430
Publication Date: 2022.08.23 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH011430. The invention thus relates to the plants, seeds and tissue cultures of the variety CH011430, and to methods for producing a corn plant produced by crossing a corn plant of variety CH011430 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH011430.