Hybrid Corn CH035643 Breeding Using Cytoplasmic Male Sterility

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current corn breeding methods face challenges in developing uniform hybrid plants with desirable traits such as high yield, disease resistance, and uniform germination, as they often result in non-uniform and unpredictable performance due to genetic differences between cross-pollinated plants.

Innovation Solution

The development of the hybrid corn variety CH035643, which incorporates a cytoplasmic or nuclear factor for male sterility and introduces specific genetic loci for traits like herbicide resistance, insect resistance, and disease resistance, using backcrossing and genetic transformation techniques to ensure consistent expression of desired characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional cross-pollination breeding methods are used, then genetic diversity is achieved, but uniformity and predictability of hybrid performance deteriorates

Engineering Contradiction:
Improvegenetic diversityVSAvoiduniformity of hybrid performance
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The patent applies homogeneity by using cytoplasmic male sterility (CMS) to ensure all plants in the hybrid population have identical cytoplasmic genetics. This uniformity in the maternal cytoplasm, combined with controlled nuclear gene inheritance, produces uniform hybrid offspring with predictable performance characteristics, directly resolving the contradiction between genetic diversity and uniformity.

Inventive Principle:
Principle #33Homogeneity

Solution Approach 2:

The patent segments the genetic inheritance system into cytoplasmic and nuclear components. By controlling which parent contributes cytoplasm (through CMS) and which contributes nuclear genes, the breeding method creates distinct genetic compartments that can be independently managed, ensuring uniformity in the hybrid population while maintaining genetic diversity through selective breeding.

Inventive Principle:
Principle #1Segmentation

2Manufacturing precision

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is achieved, but time to develop stable varieties increases

Engineering Contradiction:
Improvegenetic uniformityVSAvoidtime to develop stable varieties
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-establishing homozygous inbred lines through multiple generations of self-pollination before the hybridization step. This preliminary development of genetically uniform parent lines ensures that when crossing occurs, the F1 hybrids will be uniform without requiring additional generations of inbreeding, thus reducing the overall time to develop stable varieties.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses cytoplasmic male sterility as an intermediary mechanism to facilitate hybridization between homozygous inbred lines. The CMS trait acts as a mediator that prevents self-pollination in the female parent, ensuring cross-pollination occurs and producing uniform F1 hybrids, thereby accelerating the development of stable varieties.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If cross-pollination is used to create hybrid plants, then desirable traits from different parents are combined, but genetic consistency among progeny deteriorates

Engineering Contradiction:
Improvecombination of desirable traitsVSAvoidgenetic consistency among progeny
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent applies homogeneity by ensuring all F1 hybrid progeny receive identical cytoplasm from the CMS female parent and controlled nuclear genes from both parents. This results in genetically consistent progeny that uniformly express the desired combination of traits from both parental lines, resolving the contradiction between trait combination and genetic consistency.

Inventive Principle:
Principle #33Homogeneity

4Manufacturing precision

If male sterility factors are introduced to prevent self-pollination, then hybrid uniformity is improved, but device complexity increases

Engineering Contradiction:
Improvehybrid uniformityVSAvoidbreeding system complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The patent applies self-service by using cytoplasmic male sterility to make the female parent automatically prevent self-pollination. The CMS trait causes the plant to be sterile on its own without requiring external emasculation or manual intervention, thereby improving hybrid uniformity while minimizing the complexity of the breeding system.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS7807897B2Plants and seeds of hybrid corn variety CH035643
Publication Date: 2010.10.05 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH035643. The invention thus relates to the plants, seeds and tissue cultures of the variety CH035643, and to methods for producing a corn plant produced by crossing a corn plant of variety CH035643 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH035643.