Hybrid Corn CH048988 Breeding via Segmentation and CMS

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity in cross-pollinated plants, requiring the development of stable inbred plants and specific breeding methods like pedigree breeding and recurrent selection.

Innovation Solution

The development of the hybrid corn variety CH048988, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, using cytoplasmic-male sterility (CMS) and restorer genes, and genetic transformation techniques to introduce desired traits, along with tissue culture methods for regenerating plants with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity is improved, but genetic uniformity deteriorates

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing uniform inbred lines through self-pollination (achieving genetic uniformity), then crossing these inbreds to create hybrids (introducing genetic diversity). This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Inbred parental lines are developed and stabilized through multiple generations of self-pollination before the hybrid crossing step. This preliminary action ensures that the parental lines have the desired genetic uniformity and purity, which then enables the hybrid to achieve both uniformity (from the inbred parents) and diversity (from the cross).

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If inbred plants are developed through self-pollination, then genetic uniformity is improved, but adaptability deteriorates

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Self-pollination and selection are performed as preliminary actions to develop stable inbred parental lines with high genetic uniformity. These inbreds serve as the foundation for subsequent hybrid crosses, which then provide the genetic diversity and adaptability needed for commercial production.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding program is divided into separate segments: one for developing inbred lines (prioritizing uniformity) and another for creating hybrids (prioritizing diversity and adaptability). This segmentation allows each segment to fulfill its specific function without compromise.

Inventive Principle:
Principle #1Segmentation

3Reliability

If pedigree breeding and recurrent selection are used, then desirable traits are improved, but breeding complexity increases

Engineering Contradiction:
Improvedesirable traitsVSAvoidbreeding complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The breeding system uses self-pollination of inbred lines, which automatically maintains genetic uniformity without requiring complex intervention. The male-sterility system also self-services by automatically preventing self-pollination in hybrids, eliminating the need for manual emasculation and reducing breeding complexity.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The male-sterility system acts as an intermediary mechanism that facilitates hybrid seed production without requiring complex manual intervention. By using cytoplasmic male sterility, the system automatically ensures cross-pollination occurs, simplifying the breeding process while maintaining reliability of trait inheritance.

Inventive Principle:
Principle #24Intermediary (Mediator)

4Productivity

If male sterility systems are implemented, then hybrid seed production efficiency is improved, but genetic stability may deteriorate

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The male-sterility system serves as an intermediary that facilitates efficient hybrid seed production through automatic prevention of self-pollination. The restorer gene acts as a counterbalancing intermediary that ensures genetic stability by restoring fertility in the hybrid generation, thus maintaining both productivity and genetic stability.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9629320B2Plants and seeds of hybrid corn variety CH048988
Publication Date: 2017.04.25 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH048988. The invention thus relates to the plants, seeds and tissue cultures of the variety CH048988, and to methods for producing a corn plant produced by crossing a corn plant of variety CH048988 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH048988.