Hybrid Corn CH100368 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH100368, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with specific traits like herbicide resistance and disease resistance, achieved through genetic transformation and backcrossing methods, allowing for controlled cross-pollination and stable hybrid seed production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional self-pollination breeding methods are used, then genetic uniformity can be maintained, but hybrid vigor and yield improvement are limited

Engineering Contradiction:
ImproveyieldVSAvoidgenetic uniformity
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent segments the breeding process into distinct parental lines (inbred lines) that are genetically uniform within themselves but differ from each other. By maintaining separate pure lines and controlling their cross-pollination, the system achieves both genetic uniformity within lines and hybrid vigor in offspring, resolving the contradiction between maintaining uniformity and improving yield through hybridization.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs cytoplasmic male sterility as an intermediary mechanism to control pollination. This biological mediator prevents self-pollination in the female parent while allowing controlled cross-pollination with the male parent, ensuring genetic uniformity in the F1 hybrid generation while achieving hybrid vigor and yield improvement.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If cross-pollination is allowed freely, then genetic diversity and hybrid vigor are achieved, but self-pollination contamination reduces breeding efficiency

Engineering Contradiction:
Improvebreeding efficiencyVSAvoidpollination control
Core Design Contradiction:
ProductivityVSReliability

Solution Approach 1:

The patent introduces cytoplasmic male sterility as a biological intermediary that reliably controls pollination. The sterile female parent cannot self-pollinate, and the restorer male parent ensures cross-pollination occurs. This intermediary mechanism provides reliable pollination control while maintaining high breeding efficiency, eliminating the need for manual emasculation and reducing contamination risks.

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent employs self-service mechanisms where the plant's own genetic system controls pollination. The cytoplasmic male sterility trait automatically prevents self-pollination in female parents, and the restorer genes in male parents automatically enable cross-pollination. This self-regulating system improves breeding efficiency by eliminating the need for human intervention in emasculation and pollination control.

Inventive Principle:
Principle #25Self-service

3Productivity

If multiple breeding generations are developed, then desirable traits are combined, but time to market and development costs increase

Engineering Contradiction:
Improvetrait combination efficiencyVSAvoidbreeding cycle time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by pre-developing and characterizing inbred parental lines with specific desirable traits before hybridization. The parental lines are thoroughly evaluated for disease resistance, yield potential, and other traits in advance. This preliminary preparation allows the F1 hybrid to inherit combined traits without requiring additional generation cycles for trait combination, significantly reducing breeding cycle time while maintaining efficient trait combination.

Inventive Principle:
Principle #10Preliminary action

4Reliability

If manual emasculation is used to prevent self-pollination, then cross-pollination control is achieved, but labor costs and operational complexity increase

Engineering Contradiction:
Improveself-pollination preventionVSAvoidbreeding operation complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent uses cytoplasmic male sterility as a biological intermediary that replaces manual emasculation. The sterile female parent naturally prevents self-pollination through its genetic makeup, eliminating the need for manual removal of anthers. This intermediary mechanism maintains reliable self-pollination prevention while dramatically reducing operational complexity and labor requirements, as the plant's own genetics perform the emasculation function.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS10736291B1Plants and seeds of hybrid corn variety CH100368
Publication Date: 2020.08.11 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH100368. The invention thus relates to the plants, seeds and tissue cultures of the variety CH100368, and to methods for producing a corn plant produced by crossing a corn plant of variety CH100368 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH100368.