Hybrid Corn CH100429 Breeding via Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of trait introduction and hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH100429, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with tissue culture methods for regenerating plants with specific traits, enables the introduction of traits like herbicide resistance, disease resistance, and improved agronomic characteristics, and allows for controlled cross-pollination to produce hybrid seeds.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional self-pollination breeding methods are used, then genetic uniformity can be maintained, but self-pollination prevents efficient introduction of new traits and limits hybrid seed production

Engineering Contradiction:
Improvetrait introduction efficiencyVSAvoidgenetic stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The invention segments the breeding population into distinct inbred parental lines with specific desirable traits, allowing controlled cross-pollination between these segments to introduce new trait combinations while maintaining genetic stability within each parental line

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent uses male-sterile inbred lines as intermediaries that cannot self-pollinate, forcing cross-pollination with other inbred lines. This intermediary approach ensures efficient trait introduction through hybridization while maintaining the genetic stability of the parental lines

Inventive Principle:
Principle #24Intermediary (Mediator)

2Adaptability or versatility

If cross-pollination is used to introduce new traits, then genetic diversity and trait variety increase, but maintaining genetic stability and uniformity becomes more difficult

Engineering Contradiction:
Improvetrait varietyVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The invention performs preliminary self-pollination and selection to create homozygous inbred parental lines with stable, uniform genetics before cross-pollination. This preliminary action ensures that when cross-pollination occurs to introduce trait variety, the resulting hybrids maintain high uniformity because the parental lines are genetically stabilized

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent applies different breeding strategies to different parts of the breeding program: intensive selfing and selection to achieve local genetic uniformity in parental lines, then controlled cross-pollination to achieve local trait diversity in hybrids, with each stage optimized for its specific goal

Inventive Principle:
Principle #3Local quality

3Reliability

If extensive breeding programs are implemented to develop superior inbred plants, then hybrid quality and desirable characteristics improve, but breeding time and resource requirements increase

Engineering Contradiction:
Improvehybrid qualityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The invention employs self-sterilizing inbred lines that automatically prevent self-pollination through male sterility or self-incompatibility mechanisms. This self-service feature eliminates the need for manual emasculation and ensures automatic cross-pollination, significantly reducing breeding time and labor while maintaining high hybrid quality

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The patent changes the reproductive parameter of parental lines by inducing male sterility or self-incompatibility, transforming them from self-pollinating to obligate cross-pollinating plants. This parameter change accelerates the breeding cycle by eliminating selfing generations and directly producing uniform hybrids with desirable traits

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS11058077B2Plants and seeds of hybrid corn variety CH100429
Publication Date: 2021.07.13 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH100429. The invention thus relates to the plants, seeds and tissue cultures of the variety CH100429, and to methods for producing a corn plant produced by crossing a corn plant of variety CH100429 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH100429.