Hybrid Corn CH101353 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH101353, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with specific traits like herbicide resistance and disease resistance, achieved through genetic transformation and backcrossing techniques, allowing for controlled cross-pollination and stable hybrid seed production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If physical emasculation is used to prevent self-pollination, then hybrid seed production is achieved, but labor intensity and production cost increase

Engineering Contradiction:
Improveprevention of self-pollinationVSAvoidbreeding efficiency
Core Design Contradiction:
ReliabilityVSProductivity

Solution Approach 1:

The patent replaces mechanical emasculation with a biological system - cytoplasmic male sterility (CMS). The CMS trait genetically prevents pollen formation, eliminating the need for manual or mechanical removal of male organs. This biological substitution大幅 reduces labor intensity while maintaining reliable prevention of self-pollination in hybrid seed production

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The CMS system enables the plant to automatically prevent self-pollination through its own genetic mechanism. The sterile cytoplasm inherently prevents pollen formation without external intervention, making the plant self-sufficient in preventing unwanted self-fertilization and eliminating the need for labor-intensive emasculation procedures

Inventive Principle:
Principle #25Self-service

2Adaptability or versatility

If multiple desirable traits are combined in hybrid corn, then agronomic quality improves, but genetic stability becomes difficult to maintain

Engineering Contradiction:
Improveagronomic qualityVSAvoidgenetic stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent segments the genetic material into two distinct components: CMS cytoplasm (from one parent) and nuclear genes (from both parents). This segmentation allows the CMS trait to be stably inherited through the maternal line while the nuclear genes provide the desired agronomic traits. The clear separation maintains genetic stability while enabling combination of multiple desirable characteristics

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent applies local quality by assigning different functions to different genetic compartments. The cytoplasm specifically provides the male sterility function, while the nuclear genome provides agronomic traits such as yield, disease resistance, and stress tolerance. This functional differentiation allows each component to be optimized independently while maintaining overall genetic stability

Inventive Principle:
Principle #3Local quality

3Manufacturing precision

If inbred plants are developed through self-pollination, then uniformity is achieved, but genetic diversity decreases

Engineering Contradiction:
ImproveuniformityVSAvoidgenetic diversity
Core Design Contradiction:
Manufacturing precisionVSAdaptability or versatility

Solution Approach 1:

The patent creates asymmetry in the breeding process by using unidirectional cross-pollination from CMS female to fertile male. The CMS parent contributes only cytoplasm (maternal inheritance), while the male parent contributes nuclear genes. This asymmetric inheritance pattern ensures uniform hybrid progeny with consistent CMS trait expression, while the combination of genetically distinct parents maintains high genetic diversity in the nuclear genome

Inventive Principle:
Principle #4Asymmetry

Data Source

PatentUS20210000052A1Plants and seeds of hybrid corn variety ch101353
Publication Date: 2021.01.07 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH101353. The invention thus relates to the plants, seeds and tissue cultures of the variety CH101353, and to methods for producing a corn plant produced by crossing a corn plant of variety CH101353 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH101353.