Hybrid Corn CH101454 Cytoplasmic Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH101454, which incorporates genetic modifications and cytoplasmic male sterility to prevent self-pollination, along with specific traits like herbicide resistance and disease resistance, achieved through genetic transformation and backcrossing techniques, allowing for controlled cross-pollination and stable hybrid seed production.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional self-pollination breeding is used, then genetic uniformity is achieved, but hybrid vigor and combined desirable traits cannot be obtained

Engineering Contradiction:
Improvegenetic uniformityVSAvoidhybrid vigor
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The invention divides the breeding process into distinct segments: developing separate homozygous inbred lines with specific desirable traits, then crossing them to produce hybrid progeny. This segmentation allows maintaining genetic stability in parent lines while achieving hybrid vigor in the offspring, resolving the contradiction between uniformity and adaptability.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention merges genetically distinct homozygous inbred lines through controlled cross-pollination to create hybrid corn plants that exhibit heterosis. By combining different genetic backgrounds while maintaining homozygosity in parent lines, the invention achieves both genetic stability in breeding and hybrid vigor in production.

Inventive Principle:
Principle #5Merging (Combining)

2Adaptability or versatility

If cross-pollination is used to develop hybrids, then hybrid vigor is achieved, but self-pollination control becomes difficult

Engineering Contradiction:
Improvehybrid vigorVSAvoidpollination control
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The invention employs cytoplasmic male sterility (CMS) systems where the female parent naturally prevents self-pollination through genetic mechanisms. This self-service approach eliminates the need for manual emasculation and complex pollination control devices, reducing operational complexity while ensuring controlled cross-pollination.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The invention uses CMS cytoplasm as an intermediary mechanism to control pollination. The cytoplasmic factor acts as a natural mediator that prevents self-pollination in the female parent, facilitating controlled cross-pollination without requiring complex mechanical or manual intervention systems.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Manufacturing precision

If manual pollination control is implemented, then cross-pollination accuracy is improved, but labor intensity and cost increase

Engineering Contradiction:
Improvecross-pollination accuracyVSAvoidseed production efficiency
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The CMS system enables the plant to self-regulate pollination control, eliminating the need for labor-intensive manual emasculation and pollination procedures. This dramatically increases seed production efficiency while maintaining high cross-pollination accuracy through the natural genetic mechanism.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The invention replaces manual mechanical pollination control with a biological genetic mechanism (CMS). This substitution eliminates labor-intensive operations while maintaining precise control over cross-pollination, thereby improving productivity without sacrificing accuracy.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Stability of the object's composition

If inbred lines are developed through multiple generations of selfing, then homozygosity is achieved, but time to maturity increases

Engineering Contradiction:
ImprovehomozygosityVSAvoidbreeding cycle duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The invention performs preliminary development of homozygous inbred lines with specific desirable traits before the hybridization stage. By preparing stable parent lines in advance through systematic selfing and selection, the breeding cycle is optimized, reducing overall time to market while ensuring genetic stability.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The invention employs dynamic breeding strategies where the number of selfing generations is optimized based on the specific traits being developed. This flexible approach balances the need for homozygosity with time constraints, allowing accelerated breeding cycles while maintaining genetic stability in parent lines.

Inventive Principle:
Principle #15Dynamics

Data Source

PatentUS10856485B1Plants and seeds of hybrid corn variety CH101454
Publication Date: 2020.12.08 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH101454. The invention thus relates to the plants, seeds and tissue cultures of the variety CH101454, and to methods for producing a corn plant produced by crossing a corn plant of variety CH101454 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH101454.