Hybrid Corn CH101474 Breeding via Male Sterility

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the introduction of new genetic modifications and traits.

Innovation Solution

The development of the hybrid corn variety CH101474, which incorporates cytoplasmic or nuclear factors for male sterility, genetic modifications for herbicide resistance, disease resistance, and altered metabolic traits, along with a method for controlled cross-pollination using emasculation and self-incompatibility systems to ensure genetic diversity and trait introduction.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If self-pollination is prevented through male sterility systems, then genetic diversity and trait introduction are improved, but device complexity and breeding process complexity increase

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies preliminary action by pre-establishing male sterility systems (cytoplasmic male sterility or nuclear male sterility genes) in the parental lines before crossing. This preliminary genetic modification ensures that self-pollination is prevented from the outset, allowing controlled cross-pollination to occur naturally without requiring manual emasculation procedures, thus maintaining genetic diversity while managing breeding process complexity

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent utilizes self-service by employing self-incompatibility systems where the plant itself prevents self-pollination through genetic mechanisms. The male sterile lines or self-incompatible genotypes automatically prevent self-fertilization, eliminating the need for external intervention (such as manual emasculation or isolation) to ensure cross-pollination, thereby promoting genetic diversity without proportionally increasing breeding process complexity

Inventive Principle:
Principle #25Self-service

2Productivity

If hybrid varieties are developed through cross-pollination, then yield and disease resistance are improved, but maintaining genetic stability becomes more difficult

Engineering Contradiction:
ImproveyieldVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies segmentation by dividing the breeding program into distinct homozygous inbred parental lines with specific desirable traits (high yield, disease resistance) that are then crossed to produce the hybrid. This segmentation allows each parent to be genetically stabilized independently through multiple generations of selfing, ensuring that the hybrid combines stable, predictable traits from both parents while maintaining genetic stability through standardized parental lines

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs copying by creating and maintaining standardized homozygous inbred parental lines that can be repeatedly used to produce identical hybrid offspring. These inbred parents serve as stable genetic copies that ensure consistency across multiple hybrid production cycles, allowing the same high-yielding, disease-resistant traits to be replicated reliably in each generation of hybrids

Inventive Principle:
Principle #26Copying

3Manufacturing precision

If emasculation and controlled cross-pollination are used, then uniformity and trait introduction are improved, but productivity and breeding efficiency decrease

Engineering Contradiction:
ImproveuniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The patent applies preliminary action by pre-modifying the parental lines with male sterility genes or cytoplasmic male sterility traits before the crossing process. This preliminary genetic setup eliminates the need for time-consuming manual emasculation operations during pollination, as the male sterile parents cannot self-pollinate and require only natural or assisted cross-pollination from the other parent, thereby maintaining uniformity while significantly improving breeding efficiency

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent replaces the mechanical system of manual emasculation (physical removal of anthers) with a genetic system (male sterility genes or cytoplasmic male sterility) that prevents self-pollination automatically. This substitution eliminates the labor-intensive mechanical emasculation step while ensuring controlled cross-pollination, thereby maintaining uniformity in the hybrid progeny while dramatically improving breeding efficiency and reducing production time

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS10785949B1Plants and seeds of hybrid corn variety CH101474
Publication Date: 2020.09.29 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH101474. The invention thus relates to the plants, seeds and tissue cultures of the variety CH101474, and to methods for producing a corn plant produced by crossing a corn plant of variety CH101474 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH101474.