Hybrid Corn CH175075 Uniformity via Tissue Culture

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniformity, as existing methods often result in unpredictable performance due to genetic non-uniformity among hybrid plants.

Innovation Solution

The development of the hybrid corn variety CH175075, which incorporates cytoplasmic or nuclear factors for male sterility and desired traits like herbicide resistance, insect resistance, and disease resistance, using genetic loci introduced through backcrossing or genetic transformation, and tissue culture techniques to regenerate plants with specific physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If traditional breeding methods are used to develop hybrid corn varieties, then corn breeding can be performed, but the resulting hybrid plants exhibit genetic non-uniformity and unpredictable performance

Engineering Contradiction:
Improveperformance uniformityVSAvoidgenetic uniformity
Core Design Contradiction:
ReliabilityVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary action by developing and utilizing homozygous inbred parent lines before creating the hybrid. The inbred lines are produced through multiple generations of self-pollination and selection to achieve genetic uniformity and stability. This preliminary development of stable, uniform parent lines ensures that the resulting F1 hybrid exhibits consistent and predictable performance, resolving the contradiction between genetic uniformity and performance reliability.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is achieved, but the process requires multiple generations and careful selection

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding cycle duration
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The patent employs copying by utilizing tissue culture techniques to produce clones of selected inbred plants. Instead of requiring multiple generations of self-pollination and phenotypic selection to replicate desired traits, tissue culture creates genetic copies (clones) that faithfully reproduce the parent plant's uniform genetics and desired characteristics. This significantly reduces the time required to achieve and replicate genetic uniformity while maintaining the stability of the inbred line.

Inventive Principle:
Principle #26Copying

3Adaptability or versatility

If cross-pollination is used to create hybrid plants, then genetic diversity is introduced, but the resulting population is non-uniform and performance is unpredictable

Engineering Contradiction:
Improvegenetic diversityVSAvoidperformance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies local quality by creating a controlled cross between two specific homozygous inbred lines (I226211 and CV397654) that each possess specific desirable traits. The cross is designed to combine complementary characteristics: I226211 provides certain resistance traits and growth characteristics, while CV397654 contributes other resistance traits and agronomic qualities. This targeted combination produces an F1 hybrid with uniform genetics and predictable performance, as both parents contribute known, stable genetic backgrounds.

Inventive Principle:
Principle #3Local quality

4Reliability

If desired traits like herbicide resistance and disease resistance are introduced through backcrossing or genetic transformation, then trait specificity is improved, but the breeding complexity increases

Engineering Contradiction:
Improvetrait consistencyVSAvoidbreeding program complexity
Core Design Contradiction:
ReliabilityVSDevice complexity

Solution Approach 1:

The patent utilizes tissue culture as an intermediary technique to simplify the introduction of desired traits. Instead of requiring complex multi-generation backcrossing programs to stabilize transgenic or mutated traits, tissue culture serves as a mediator that can rapidly propagate plants with introduced traits. The tissue culture system maintains genetic stability and uniformity while reducing the overall breeding complexity by providing a controlled environment for trait expression and propagation.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS8940977B2Plants and seeds of hybrid corn variety CH175075
Publication Date: 2015.01.27 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH175075. The invention thus relates to the plants, seeds and tissue cultures of the variety CH175075, and to methods for producing a corn plant produced by crossing a corn plant of variety CH175075 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH175075.