Hybrid Corn CH184535 Breeding via Segmentation and Inversion

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH184535, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with a process for crossing parent plants to produce hybrid seeds, utilizing genetic loci that confer resistance to herbicides, diseases, and other desirable characteristics, and tissue cultures capable of regenerating plants with specific morphological and physiological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Stability of the object's composition

If traditional self-pollination breeding methods are used, then genetic uniformity is achieved, but hybrid vigor and desired trait combination are limited

Engineering Contradiction:
Improvegenetic uniformityVSAvoidhybrid vigor
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The breeding process is segmented into distinct phases: developing pure inbred lines through self-pollination to achieve genetic uniformity, then crossing these lines to produce hybrids that exhibit heterosis. This segmentation allows simultaneous achievement of genetic stability in parent lines and hybrid vigor in offspring.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Instead of directly breeding for hybrid traits through complex cross-pollination from the start, the method inverts the approach by first establishing genetically uniform inbred lines through self-pollination, then using these standardized lines as parents to generate hybrids. This reversal simplifies the breeding process and ensures consistent hybrid performance.

Inventive Principle:
Principle #13The other way round (Inversion)

2Adaptability or versatility

If cross-pollination is used to produce hybrids, then hybrid vigor and trait diversity are improved, but self-pollination control and genetic stability become difficult to maintain

Engineering Contradiction:
Improvehybrid vigorVSAvoidgenetic stability
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

Before producing hybrids through cross-pollination, the method performs preliminary action by developing and stabilizing pure inbred parent lines through multiple generations of self-pollination and selection. This preliminary genetic stabilization ensures that when cross-pollination occurs, the hybrids inherit predictable and stable traits from well-characterized parents.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The method creates true-breeding inbred lines that serve as standardized genetic copies or templates. These inbred parents can be repeatedly used to produce identical hybrid combinations, ensuring genetic stability and reproducibility across multiple hybrid production cycles.

Inventive Principle:
Principle #26Copying

3Adaptability or versatility

If extensive breeding programs are implemented to combine multiple desirable traits, then trait diversity and yield potential are improved, but breeding complexity and time requirements increase

Engineering Contradiction:
Improvetrait diversityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The breeding method develops inbred parent lines that serve multiple functions: they are genetically uniform for stability, can be crossed to produce hybrid vigor, and can be selected for specific traits like disease resistance or yield. These multi-functional inbred lines simplify the overall breeding program by serving as versatile building blocks for different hybrid combinations.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The method changes the key parameter from direct hybrid breeding complexity to simplified inbred line development. By focusing breeding efforts on stabilizing a few key inbred parent lines with desired traits, then using standardized crossing protocols, the program reduces overall complexity while maintaining ability to produce diverse hybrid combinations.

Inventive Principle:
Principle #35Parameter changes

4Manufacturing precision

If manual emasculation and cross-pollination control are used, then hybrid seed purity is improved, but labor intensity and production efficiency decrease

Engineering Contradiction:
Improvehybrid seed purityVSAvoidseed production efficiency
Core Design Contradiction:
Manufacturing precisionVSProductivity

Solution Approach 1:

The method employs self-service mechanisms where the breeding system itself ensures hybrid purity without extensive manual intervention. This includes using male-sterile inbred lines that naturally prevent self-pollination, or using timing differences in pollen shed and silk emergence to ensure cross-pollination occurs between different parent lines, thereby maintaining hybrid seed purity through biological self-regulation rather than manual emasculation.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS10448599B1Plants and seeds of hybrid corn variety CH184535
Publication Date: 2019.10.22 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH184535. The invention thus relates to the plants, seeds and tissue cultures of the variety CH184535, and to methods for producing a corn plant produced by crossing a corn plant of variety CH184535 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH184535.