Hybrid Corn CH199546 Breeding via Cytoplasmic-Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH199546, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility and transgenes integrated at specific chromosomal locations, along with tissue culture techniques for regenerating plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and performance unpredictability worsen

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance uniformity
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The patent applies preliminary action by first developing and selecting homozygous inbred plants through multiple generations of self-pollination and selection before performing the final cross. This preliminary selection process ensures that the parent plants are genetically uniform and stable, which then produces uniform F1 hybrid progeny when crossed, thereby resolving the contradiction between trait combination and performance uniformity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent utilizes parameter changes by transitioning from heterozygous parents to homozygous inbred lines through controlled self-pollination over multiple generations. This changes the genetic parameter from heterozygosity to homozygosity, creating stable inbred plants that when crossed produce uniform hybrids, thus achieving both trait combination and performance reliability.

Inventive Principle:
Principle #35Parameter changes

2Reliability

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is improved, but the time to develop stable varieties worsens

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent applies preliminary action by conducting multiple generations of self-pollination and selection in advance to develop stable homozygous inbred plants before the final hybridization. This preliminary work, though time-consuming, ensures genetic uniformity and stability that cannot be achieved without such extended selection periods.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs continuity of useful action through repeated cycles of self-pollination, selection, and regeneration over multiple generations. This continuous process of selfing and selecting desirable traits ensures that the inbred lines achieve complete homozygosity and stability, which is necessary for producing uniform hybrids.

Inventive Principle:
Principle #20Continuity of useful action

3Adaptability or versatility

If traditional breeding methods are used to develop hybrid varieties, then genetic diversity is improved, but manufacturing complexity and cost worsen

Engineering Contradiction:
Improvegenetic diversityVSAvoidbreeding process complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent applies segmentation by dividing the breeding process into distinct phases: (1) development of homozygous inbred lines through self-pollination and selection, (2) crossing of selected inbreds to produce F1 hybrids, and (3) evaluation and selection of superior hybrids. This segmentation simplifies the overall complex breeding process by breaking it down into manageable, systematic steps that can be controlled and standardized.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent utilizes parameter changes by systematically transitioning from diverse heterozygous populations to uniform homozygous inbred lines, and then to uniform F1 hybrids. This controlled change in genetic parameters (from heterozygosity to homozygosity) simplifies the breeding process by creating predictable, uniform outcomes at each stage, reducing the complexity of managing genetic diversity.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS8692056B2Plants and seeds of hybrid corn variety CH199546
Publication Date: 2014.04.08 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH199546. The invention thus relates to the plants, seeds and tissue cultures of the variety CH199546 , and to methods for producing a corn plant produced by crossing a corn plant of variety CH199546 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH199546.