Hybrid Corn CH203254 Uniformity via Cytoplasmic Male Sterility

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniformity, as existing methods often result in unpredictable performance due to genetic non-uniformity among hybrid plants.

Innovation Solution

The development of the hybrid corn variety CH203254, which incorporates cytoplasmic or nuclear factors for male sterility and desired traits like herbicide resistance, insect resistance, and disease resistance, using genetic loci introduced through backcrossing or genetic transformation, and tissue culture techniques to regenerate plants with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If traditional breeding methods are used to develop hybrid corn varieties, then genetic diversity and trait combination are improved, but uniformity and predictability of hybrid performance deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoiduniformity
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The breeding process is segmented into distinct phases: developing homozygous inbred parent lines through multiple generations of self-pollination, then crossing these standardized parents to produce uniform F1 hybrids. This segmentation ensures genetic stability in parents while achieving desired trait combinations in hybrids.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs cytoplasmic male sterility (CMS) as a genetic parameter change to prevent self-pollination and ensure uniform cross-pollination. By introducing specific cytoplasmic factors that confer male sterility, the system achieves uniform hybrid production with predictable performance while maintaining genetic diversity through controlled crossing.

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If self-pollination is used to develop homozygous inbred plants, then uniformity of parent lines is improved, but genetic diversity and adaptability deteriorate

Engineering Contradiction:
Improveuniformity of parent linesVSAvoidgenetic diversity
Core Design Contradiction:
Manufacturing precisionVSAdaptability or versatility

Solution Approach 1:

Homozygous inbred parent lines are developed in advance through multiple generations of self-pollination and selection before the hybrid crossing stage. This preliminary action ensures uniform, stable parent lines with known genetic characteristics, which are then crossed to introduce genetic diversity in the hybrid generation.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Cytoplasmic male sterility acts as an intermediary mechanism that enables controlled genetic diversity. The CMS factor prevents self-pollination in the hybrid generation, forcing cross-pollination between different inbred lines, thereby introducing genetic diversity while maintaining uniformity through standardized parent lines.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If cross-pollination is used to produce hybrid plants, then genetic diversity and trait combination are improved, but uniformity of hybrid population deteriorates

Engineering Contradiction:
Improvegenetic diversityVSAvoiduniformity of hybrid population
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The patent applies local quality by ensuring that specific genetic characteristics (homozygosity) are maintained in the parent lines, while allowing genetic diversity in the hybrid population through controlled cross-pollination. The standardized genetic background of parents provides uniformity, while the cross-pollination event introduces desired genetic variation.

Inventive Principle:
Principle #3Local quality

Solution Approach 2:

The hybrid corn variety combines genetic material from two distinct homozygous inbred parent lines, creating a composite genetic structure. This composite approach allows the hybrid to inherit desirable traits from both parents while maintaining uniformity through the standardized genetic backgrounds of the parent lines and controlled pollination.

Inventive Principle:
Principle #40Composite materials

4Adaptability or versatility

If multiple breeding generations are conducted to combine desirable traits, then trait combination and adaptability are improved, but time to maturity and development cycle increase

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding cycle duration
Core Design Contradiction:
Adaptability or versatilityVSLoss of time

Solution Approach 1:

Inbred parent lines are developed in advance and maintained as stable, standardized genetic resources. This preliminary development allows rapid hybrid production without repeating the lengthy inbreeding process, significantly reducing the breeding cycle time while maintaining trait combination capabilities.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent establishes a buffer of pre-developed, characterized inbred lines with known genetic properties and desirable traits. This cushion of genetic resources allows breeders to quickly assemble hybrid combinations without time-consuming development, maintaining adaptability while reducing development cycle duration.

Inventive Principle:
Principle #11Beforehand cushioning (Prior cushioning)

Data Source

PatentUS9018476B2Plants and seeds of hybrid corn variety CH203254
Publication Date: 2015.04.28 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH203254. The invention thus relates to the plants, seeds and tissue cultures of the variety CH203254, and to methods for producing a corn plant produced by crossing a corn plant of variety CH203254 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH203254.