Hybrid Corn CH286065 Breeding for Uniform Trait Expression

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid plants with desirable traits such as high yield, disease resistance, and uniformity, as they often result in unpredictable performance due to genetic non-uniformity among hybrid plants.

Innovation Solution

The development of the hybrid corn variety CH286065, which incorporates cytoplasmic or nuclear factors for male sterility and desired traits like herbicide resistance, insect resistance, and disease resistance, using genetic transformation techniques and backcrossing methods to introduce specific genetic loci, ensuring consistent performance and uniformity.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If conventional breeding techniques are used to develop hybrid corn, then genetic diversity is achieved, but uniformity and predictability of performance deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoiduniformity of hybrid plants
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The breeding program is segmented into distinct phases: developing homozygous inbred lines through repeated selfing, maintaining these lines as separate uniform genetic units, and then crossing them in controlled manner. This segmentation ensures that genetic diversity is achieved through the cross while each parent contributes uniform, predictable genetic material.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent employs cytoplasmic male sterility (CMS) as a parameter change to control pollination. By introducing a cytoplasmic factor that prevents self-pollination, the system ensures uniform cross-pollination between designated parent lines, eliminating variability from unintended selfing or random mating.

Inventive Principle:
Principle #35Parameter changes

2Manufacturing precision

If self-pollination is used to develop inbred lines, then uniformity within lines is improved, but time required for breeding increases

Engineering Contradiction:
Improveuniformity of inbred linesVSAvoidbreeding cycle duration
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

Inbred lines are developed and stabilized through repeated selfing before the hybridization phase. This preliminary action ensures that each parent line is genetically uniform and stable, which accelerates the overall breeding process by eliminating the need for additional uniformity checks after crossing.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent applies different breeding strategies to different components: intensive selfing and selection is applied locally to develop uniform inbred parent lines, while controlled cross-pollination is applied to generate the hybrid. This localized application of breeding methods optimizes both uniformity and efficiency.

Inventive Principle:
Principle #3Local quality

3Productivity

If cross-pollination is used to create hybrids, then genetic diversity and vigor are improved, but genetic uniformity among hybrids deteriorates

Engineering Contradiction:
Improvehybrid vigor and yieldVSAvoidgenetic uniformity of hybrid population
Core Design Contradiction:
ProductivityVSManufacturing precision

Solution Approach 1:

The patent introduces cytoplasmic male sterility as a controlling parameter to ensure uniform cross-pollination. The CMS trait prevents self-pollination and ensures that all plants receive pollen only from the designated male parent, creating genetically uniform hybrids while maintaining the benefits of cross-pollination.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The cytoplasmic male sterility factor acts as an intermediary mechanism that mediates between the need for cross-pollination (for hybrid vigor) and the need for uniformity. It ensures that cross-pollination occurs uniformly across the population without introducing genetic variability from random mating.

Inventive Principle:
Principle #24Intermediary (Mediator)

4Adaptability or versatility

If multiple desirable traits are combined in breeding, then adaptability and resistance are improved, but complexity of breeding program increases

Engineering Contradiction:
Improvedisease and pest resistanceVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent merges multiple desirable traits (herbicide resistance, insect resistance, disease resistance) into the inbred parent lines before hybridization. By combining these traits in the parents through selective breeding and crossing, the resulting hybrid inherits all traits simultaneously, simplifying the overall breeding program compared to developing each trait separately.

Inventive Principle:
Principle #5Merging (Combining)

Data Source

PatentUS9258969B2Plants and seeds of hybrid corn variety CH286065
Publication Date: 2016.02.16 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH286065. The invention thus relates to the plants, seeds and tissue cultures of the variety CH286065, and to methods for producing a corn plant produced by crossing a corn plant of variety CH286065 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH286065.