Hybrid Corn CH294149 Breeding via Segmentation and Male Sterility

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH294149, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, using cytoplasmic-male sterility and genetic transformation techniques to introduce desired traits, and tissue culture methods for regenerating plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing uniform homozygous inbred lines through self-pollination, then using controlled cross-pollination only at the final hybridization stage. This segmentation allows genetic diversity to be introduced only when needed, maintaining uniformity during the development phase.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred parent lines are developed and stabilized through multiple generations of self-pollination and selection before the actual hybrid crossing. This preliminary action ensures that when cross-pollination occurs, the parents have already achieved the desired genetic uniformity, preventing genetic unpredictability in the hybrid.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but breeding efficiency and time to develop hybrids deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding efficiency
Core Design Contradiction:
Stability of the object's compositionVSProductivity

Solution Approach 1:

Corn plants naturally self-pollinate, requiring minimal human intervention to achieve homozygosity in inbred lines. This self-service mechanism allows automatic genetic uniformity to be established over generations without complex manual pollination control, improving breeding efficiency while maintaining uniformity.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The breeding program uses periodic cycles of self-pollination for a predetermined number of generations to establish uniformity, then switches to a single cross-pollination event to create the hybrid. This periodic action pattern efficiently balances the time required for uniformity development with the need for hybrid production.

Inventive Principle:
Principle #19Periodic action

3Adaptability or versatility

If traditional breeding methods are used, then natural genetic variation is maintained, but introduction of specific desired traits deteriorates

Engineering Contradiction:
Improvenatural genetic variationVSAvoidtrait introduction efficiency
Core Design Contradiction:
Adaptability or versatilityVSEase of manufacture

Solution Approach 1:

Homozygous inbred lines serve as intermediary carriers that preserve the natural genetic background while allowing precise introduction of specific desired traits through controlled crossing. The inbred lines act as stable vehicles that maintain overall genetic variation while enabling targeted trait incorporation at the hybridization stage.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9913444B2Plants and seeds of hybrid corn variety CH294149
Publication Date: 2018.03.13 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH294149. The invention thus relates to the plants, seeds and tissue cultures of the variety CH294149, and to methods for producing a corn plant produced by crossing a corn plant of variety CH294149 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH294149.