Hybrid Corn CH334303 Breeding for Genetic Uniformity

Resolve Bottlenecks,
Find Innovative Solutions
Generate Solutions

Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity in cross-pollinated plants, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH334303, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility and transgenes integrated at a single chromosomal location, along with tissue culture methods for regenerating plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to combine desirable traits from different plants, then genetic diversity and trait combination are improved, but genetic non-uniformity and unpredictable performance occur

Engineering Contradiction:
Improvetrait combinationVSAvoidperformance uniformity
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding process is segmented into distinct phases: developing homozygous inbred lines through repeated self-pollination, then crossing these inbreds to produce uniform F1 hybrids. This segmentation ensures that each phase achieves its specific goal of either maximizing genetic diversity (inbreds) or genetic uniformity (F1 hybrids).

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are developed through preliminary self-pollination and selection before the actual hybrid crossing. This preliminary action ensures that the parental lines are genetically stable and uniform, which then produces predictable and uniform F1 hybrids when crossed.

Inventive Principle:
Principle #10Preliminary action

2Reliability

If self-pollination is used to develop homozygous inbred plants, then genetic uniformity is improved, but loss of genetic diversity occurs

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
ReliabilityVSAdaptability or versatility

Solution Approach 1:

The breeding program is divided into separate stages: first developing homozygous inbred lines through self-pollination to achieve genetic uniformity, then crossing these inbreds to restore genetic diversity in the F1 generation. Each stage serves its specific purpose without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred plants are created through preliminary self-pollination and selection over multiple generations. This preliminary action establishes genetically uniform parental lines that can then be crossed to produce diverse yet uniform F1 hybrids.

Inventive Principle:
Principle #10Preliminary action

3Ease of manufacture

If traditional breeding methods are used to develop new varieties, then existing knowledge is utilized, but development time and complexity increase

Engineering Contradiction:
Improvebreeding processVSAvoidbreeding cycle duration
Core Design Contradiction:
Ease of manufactureVSLoss of time

Solution Approach 1:

Homozygous inbred plants are developed through preliminary self-pollination and selection before hybrid crossing. This preliminary action eliminates the need for complex multi-generation breeding cycles, as the inbreds are already genetically stable and uniform when ready for crossing.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The breeding approach changes the key parameter of plant homozygosity status. By working with homozygous inbreds rather than heterogeneous populations, the breeding cycle is simplified and accelerated, as crossing inbreds produces uniform F1 hybrids in a single generation rather than requiring multiple generations of selection.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9072261B2Plants and seeds of hybrid corn variety CH334303
Publication Date: 2015.07.07 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH334303. The invention thus relates to the plants, seeds and tissue cultures of the variety CH334303, and to methods for producing a corn plant produced by crossing a corn plant of variety CH334303 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH334303.