Hybrid Corn CH337729 Breeding via Male Sterility and Segmentation

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH337729, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with tissue culture methods for regenerating plants with specific physiological and morphological characteristics, enables the production of hybrid seeds with enhanced traits like herbicide resistance and improved agronomic qualities.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional self-pollination breeding methods are used, then genetic uniformity can be maintained, but hybrid vigor and productivity are limited

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The invention divides the breeding population into distinct male and female parent lines with different genetic compositions. Male parent plants possess male fertility while female parent plants are rendered male sterile through genetic modification, creating segmented functional groups that prevent self-pollination and enable controlled cross-pollination for hybrid seed production

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The invention introduces a male sterility gene as an intermediary mechanism to control pollination. This genetic mediator prevents self-pollination in female parent plants while allowing controlled cross-pollination with male parent plants, thereby facilitating hybrid seed production without compromising genetic stability through uncontrolled selfing

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If cross-pollination is used to develop hybrid varieties, then hybrid vigor and yield are improved, but control over self-pollination becomes difficult

Engineering Contradiction:
Improvehybrid yieldVSAvoidpollination control
Core Design Contradiction:
ProductivityVSEase of operation

Solution Approach 1:

The invention extracts the male fertility function from female parent plants through genetic modification. By introducing a male sterility gene into the female parent line, the male reproductive capability is removed from plants that need to receive pollen, ensuring they cannot self-pollinate while still allowing them to be pollinated by male parent plants

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

Instead of preventing cross-pollination and allowing self-pollination (traditional approach), the invention inverts the approach by preventing self-pollination through male sterility while facilitating cross-pollination. Male parent plants are kept fertile to provide pollen, while female parent plants are made sterile to receive pollen only, reversing the conventional control mechanism

Inventive Principle:
Principle #13The other way round (Inversion)

3Adaptability or versatility

If multiple breeding traits are combined in a single variety, then agronomic quality improves, but breeding complexity increases

Engineering Contradiction:
Improveagronomic qualityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The invention creates parent lines that serve multiple functions within the breeding system. The male parent line provides both pollen for cross-pollination and carries desirable agronomic traits, while the female parent line serves as both the sterile recipient and carrier of additional desirable traits. This multi-functionality allows combination of multiple breeding traits without proportionally increasing program complexity

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The invention applies different genetic modifications to different parent lines based on their specific roles. The male parent line is modified to maintain fertility and carry specific traits, while the female parent line is modified with male sterility and carries complementary traits. This localized application of genetic modifications optimizes each parent line for its specific function while combining multiple desirable characteristics in the final hybrid

Inventive Principle:
Principle #3Local quality

Data Source

PatentUS10638681B2Plants and seeds of hybrid corn variety CH337729
Publication Date: 2020.05.05 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH337729. The invention thus relates to the plants, seeds and tissue cultures of the variety CH337729, and to methods for producing a corn plant produced by crossing a corn plant of variety CH337729 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH337729.