Hybrid Corn CH354074 Breeding Segmentation for Genetic Uniformity

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity in cross-pollinated plants, requiring the development of stable inbred plants and specific breeding methods to combine desirable characteristics.

Innovation Solution

The development of the hybrid corn variety CH354074, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, using cytoplasmic-male sterility and transgenes integrated at a single chromosomal location, along with tissue cultures capable of regenerating plants with specific physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to develop corn hybrids, then genetic diversity and trait combination are improved, but genetic uniformity and predictability of performance deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: developing homozygous inbred parent lines through self-pollination to ensure genetic uniformity, then crossing these standardized inbreds to produce hybrid offspring that exhibit uniform heterosis. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred parent lines are developed and standardized before the hybrid crossing stage. This preliminary action of creating genetically uniform parents through multiple generations of selfing ensures that when crossing occurs, the resulting hybrids will be uniform and predictable, resolving the contradiction between diversity and uniformity.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity and stability are improved, but genetic diversity and adaptability deteriorate

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

The breeding system dynamically transitions between self-pollination (for uniformity) and cross-pollination (for diversity) at different stages. Inbred lines are developed through selfing to achieve homozygosity, then these uniform lines are crossed to generate hybrid diversity, allowing the system to exploit both extremes at appropriate times.

Inventive Principle:
Principle #15Dynamics

Solution Approach 2:

The breeding approach changes the genetic parameter state from heterozygous (diverse) to homozygous (uniform) during inbred development, then reverses to heterozygous during hybrid production. This parameter change strategy allows optimization of uniformity when needed and diversity when needed.

Inventive Principle:
Principle #35Parameter changes

3Adaptability or versatility

If multiple breeding methods are used to combine desirable traits, then trait diversity is improved, but breeding complexity and time required deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent employs universal breeding methods (pedigree breeding and recurrent selection) that can be applied across different corn varieties and trait combinations. These standardized multi-functional approaches reduce the need for developing specialized complex procedures for each breeding objective.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The breeding program uses repeated copying and selection of desirable phenotypes across multiple generations and populations. By copying successful trait combinations and propagating them through standardized inbred line development, the program efficiently combines traits without requiring overly complex procedures.

Inventive Principle:
Principle #26Copying

Data Source

PatentUS8134057B2Plants and seeds of hybrid corn variety CH354074
Publication Date: 2012.03.13 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH354074. The invention thus relates to the plants, seeds and tissue cultures of the variety CH354074, and to methods for producing a corn plant produced by crossing a corn plant of variety CH354074 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH354074.