Hybrid Corn CH356005 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH356005, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, using cytoplasmic-male sterility and genetic transformation techniques, along with tissue culture methods to regenerate plants with consistent physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used in corn breeding, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing uniform inbred parental lines through self-pollination, then using controlled cross-pollination only at the hybridization stage. This segmentation allows genetic diversity to be introduced only when needed, while maintaining uniformity in the parental lines and predictable hybrid performance.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Inbred parental lines are developed and stabilized through multiple generations of self-pollination before the actual hybridization. This preliminary action ensures that the parental lines are genetically uniform and stable, which then enables predictable hybrid performance when cross-pollination occurs.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If multiple generations of self-pollination are used to develop inbred lines, then genetic uniformity is improved, but breeding time and complexity increase

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The breeding program uses periodic cycles of self-pollination for a fixed number of generations to achieve inbreeding, followed by a single cross-pollination event to produce hybrids. This periodic structure allows systematic achievement of genetic uniformity without indefinite time investment, as the inbred lines can then be maintained and reused.

Inventive Principle:
Principle #19Periodic action

Solution Approach 2:

Once uniform inbred parental lines are developed, they serve as reusable genetic templates. The same inbred lines can be crossed repeatedly to produce uniform hybrid seed, avoiding the need to re-develop the inbred lines each time hybrids are needed, thus reducing overall breeding time.

Inventive Principle:
Principle #26Copying

3Adaptability or versatility

If traditional breeding methods are used, then trait combination is improved, but yield uniformity and performance predictability deteriorate

Engineering Contradiction:
Improvetrait combinationVSAvoidyield uniformity
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The invention uses homozygous inbred parental lines that are genetically homogeneous and uniform. When these uniform parents are cross-pollinated, they produce F1 hybrid progeny that are also genetically uniform and homogeneous, ensuring consistent yield and performance across the hybrid population while still combining desired traits from both parents.

Inventive Principle:
Principle #33Homogeneity

Data Source

PatentUS8450582B2Plants and seeds of hybrid corn variety CH356005
Publication Date: 2013.05.28 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH356005. The invention thus relates to the plants, seeds and tissue cultures of the variety CH356005, and to methods for producing a corn plant produced by crossing a corn plant of variety CH356005 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH356005.