Hybrid Corn CH369747 Breeding for Genetic Uniformity

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH369747, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, using cytoplasmically-inherited traits and genetic transformation techniques to introduce desired characteristics, and a method for producing hybrid seeds through controlled cross-pollination to prevent self-pollination.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to develop corn hybrids, then genetic diversity and desirable traits can be combined, but genetic non-uniformity and unpredictability occur in the resulting hybrid population

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The breeding process is segmented into distinct stages: developing homozygous inbred parent lines through self-pollination, then using controlled cross-pollination only at the hybridization stage. This segmentation ensures genetic uniformity within each parent line while achieving desired genetic diversity in the hybrid through controlled combination of specific parental genotypes.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Homozygous inbred parent plants are developed and stabilized through multiple generations of self-pollination before the cross-pollination step. This preliminary action ensures that each parent line has uniform and predictable genetics, which then allows for uniform and predictable hybrid performance when crossed.

Inventive Principle:
Principle #10Preliminary action

2Manufacturing precision

If self-pollination is used to develop homozygous inbred plants, then uniformity and stability are achieved, but time and generations required for breeding increase

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding time
Core Design Contradiction:
Manufacturing precisionVSLoss of time

Solution Approach 1:

Multiple generations of self-pollination are performed in advance to establish homozygous inbred parent lines with uniform genetics. This preliminary action, though time-consuming, is performed only once for each parent line, and the resulting uniform parents can then be crossed repeatedly to produce uniform hybrids without requiring additional generations of self-pollination.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

Once homozygous inbred parent lines are developed, they serve as stable genetic copies that can be propagated and crossed repeatedly. The uniform genetic makeup of the inbred parents is copied into each hybrid generation, allowing consistent hybrid performance without requiring repeated time-intensive self-pollination cycles.

Inventive Principle:
Principle #26Copying

3Manufacturing precision

If controlled cross-pollination methods are implemented to prevent self-pollination, then hybrid uniformity is improved, but breeding process complexity increases

Engineering Contradiction:
Improvehybrid uniformityVSAvoidbreeding process complexity
Core Design Contradiction:
Manufacturing precisionVSDevice complexity

Solution Approach 1:

The male reproductive structures (tassels) are removed from selected parent plants to eliminate self-pollination capability. This extraction of the male function from specific plants ensures that only controlled cross-pollination can occur, simplifying the management of hybrid uniformity while maintaining breeding efficiency.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

Physical barriers such as bags are used as intermediaries to control pollen transfer between plants. These simple intermediary structures facilitate controlled cross-pollination without requiring complex mechanical or chemical systems, achieving hybrid uniformity through straightforward physical management.

Inventive Principle:
Principle #24Intermediary (Mediator)

Data Source

PatentUS9210867B2Plants and seeds of hybrid corn variety CH369747
Publication Date: 2015.12.15 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH369747. The invention thus relates to the plants, seeds and tissue cultures of the variety CH369747, and to methods for producing a corn plant produced by crossing a corn plant of variety CH369747 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH369747.