Hybrid Corn CH545762 Cytoplasmic Male Sterility Breeding

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH545762, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with specific genetic loci conferring herbicide resistance, disease resistance, and other desirable characteristics, using advanced breeding methods and genetic transformation techniques like genome editing.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If traditional self-pollination breeding methods are used, then genetic uniformity can be achieved, but hybrid seed production efficiency is limited due to self-pollination

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic uniformity
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary anti-action by introducing cytoplasmic male sterility traits that prevent self-pollination before it can occur. The CMS system ensures that female parent plants cannot produce viable pollen, thereby preemptively blocking self-pollination and forcing cross-pollination between different parental lines, which resolves the contradiction between preventing self-pollination and maintaining genetic uniformity.

Inventive Principle:
Principle #9Preliminary anti-action

Solution Approach 2:

The patent uses cytoplasmic male sterility as an intermediary mechanism to control pollination. The CMS trait acts as a mediator that prevents self-pollination while allowing controlled cross-pollination through the use of maintainer and restorer lines, thereby enabling efficient hybrid seed production while maintaining genetic uniformity in the F1 generation.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If multiple desirable traits are combined in hybrid varieties, then yield and resistance improve, but genetic stability becomes difficult to maintain

Engineering Contradiction:
ImproveyieldVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent segments the genetic material into distinct parental lines, each carrying specific desirable traits. By dividing the complex genetic requirements into separate parental genomes and using controlled cross-pollination, the patent maintains genetic stability in the F1 hybrid while combining multiple desirable traits such as yield, disease resistance, and herbicide tolerance from different parents.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The patent inverts the traditional approach by using male sterility to prevent self-pollination rather than relying on self-pollination to maintain uniformity. This inversion ensures that F1 hybrids are produced exclusively through controlled cross-pollination, maintaining genetic stability even when combining multiple complex traits from different parental lines.

Inventive Principle:
Principle #13The other way round (Inversion)

3Productivity

If cytoplasmic male sterility is used to prevent self-pollination, then hybrid seed production efficiency improves, but device complexity increases due to multiple parental lines required

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidbreeding system complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent applies universality by developing multi-functional parental lines that simultaneously serve as female parents for hybrid production and as sources of desirable agronomic traits. The CMS system is integrated with trait stacking, allowing the same breeding framework to produce hybrids with multiple desirable characteristics while maintaining operational simplicity through standardized pollination control protocols.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Data Source

PatentUS10993393B2Plants and seeds of hybrid corn variety CH545762
Publication Date: 2021.05.04 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH545762. The invention thus relates to the plants, seeds and tissue cultures of the variety CH545762, and to methods for producing a corn plant produced by crossing a corn plant of variety CH545762 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH545762.