Hybrid Corn CH576533 Breeding via Male Sterility and Backcrossing

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and avoiding self-pollination, which limits the efficiency of trait introduction and hybrid seed production.

Innovation Solution

The development of the hybrid corn variety CH576533, which incorporates cytoplasmic or nuclear factors for male sterility and genetic loci conferring traits like herbicide resistance, disease resistance, and altered metabolism, along with a method for controlled cross-pollination using emasculation and self-incompatibility systems to produce hybrid seeds with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If self-pollination is allowed in corn breeding, then genetic stability is maintained, but hybrid seed production efficiency decreases due to unwanted self-pollination

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidgenetic stability
Core Design Contradiction:
ProductivityVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary action by inducing male sterility in the female parent before pollination occurs. This prevents self-pollination in advance, ensuring that only controlled cross-pollination with the male parent can occur, thereby提高 hybrid seed production efficiency while maintaining genetic stability through controlled breeding.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent uses an interspecific hybrid (S. arvenris × S. vulgaris) as an intermediary to transfer the male sterile trait from wild mustard to cultivated Brassica species. This intermediary approach enables the introduction of self-incompatibility mechanisms that prevent self-pollination while maintaining genetic stability through controlled cross-breeding programs.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If male sterility is induced to prevent self-pollination, then hybrid seed production efficiency improves, but breeding process complexity increases

Engineering Contradiction:
Improvehybrid seed production efficiencyVSAvoidbreeding process complexity
Core Design Contradiction:
ProductivityVSDevice complexity

Solution Approach 1:

The patent extracts and utilizes the natural self-incompatibility mechanism from the interspecific hybrid (S. arvenris × S. vulgaris) to induce male sterility. By taking out this pre-existing biological mechanism rather than creating artificial sterility systems, the breeding process complexity is minimized while still achieving efficient hybrid seed production through prevented self-pollination.

Inventive Principle:
Principle #2Taking out (Extraction)

3Adaptability or versatility

If interspecific hybridization is used to introduce new traits, then genetic diversity and trait introduction improve, but genetic stability and uniformity decrease

Engineering Contradiction:
Improvetrait introduction capabilityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The patent applies preliminary action by performing repeated backcrossing (BC1, BC2, BC3 generations) to progressively restore genetic uniformity after interspecific hybridization. This systematic approach allows new traits from S. arvenris to be introduced while gradually recovering the genetic stability and uniformity of the cultivated S. vulgaris parent through multiple generations of controlled breeding.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The patent employs feedback mechanisms through phenotypic selection at each backcrossing generation, where plants exhibiting desired traits and recovering parental characteristics are selected for further breeding. This feedback-driven selection process ensures that genetic uniformity is progressively restored while retaining beneficial traits from the interspecific hybrid.

Inventive Principle:
Principle #23Feedback

Data Source

PatentUS9883649B1Plants and seeds of hybrid corn variety CH576533
Publication Date: 2018.02.06 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH576533. The invention thus relates to the plants, seeds and tissue cultures of the variety CH576533, and to methods for producing a corn plant produced by crossing a corn plant of variety CH576533 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH576533.