Hybrid Corn CH799142 Cytoplasmic Male Sterility Breeding
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Solution Overview
Problem
Current corn breeding techniques face challenges in developing hybrid varieties that combine desirable traits such as high yield, disease resistance, and uniformity, while maintaining genetic stability and preventing self-pollination, which limits the efficiency of hybrid seed production.
Innovation Solution
The development of the hybrid corn variety CH799142, which incorporates genetic modifications and cytoplasmic male sterility traits to prevent self-pollination, along with specific genetic loci conferring herbicide resistance, insect resistance, and other desirable characteristics, using advanced breeding methods and genetic transformation techniques like genome editing.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional breeding methods are used to develop hybrid corn varieties, then genetic diversity and desirable traits can be combined, but self-pollination occurs which reduces hybrid seed production efficiency
Solution Approach 1:
The patent extracts and removes the male reproductive capability from the female parent line by inducing cytoplasmic male sterility (CMS). This is achieved by introducing specific mitochondrial genes (e.g., T-urf13, CMS-T, CMS-W23) into the female parent, which prevent pollen development. As a result, the female parent cannot self-pollinate or pollinate other plants, ensuring 100% cross-pollination and hybrid seed production efficiency.
Solution Approach 2:
The patent introduces a restorer gene (Rf1 or Rf2) as an intermediary element that controls the expression of male sterility. The restorer gene acts as a mediator between the CMS cytoplasm and pollen development: when present, it restores male fertility; when absent, male sterility is expressed. This allows precise control of self-pollination prevention in different genetic backgrounds.
2Productivity
If cytoplasmic male sterility is introduced to prevent self-pollination, then hybrid seed production efficiency improves, but genetic stability and uniformity may be affected
Solution Approach 1:
The patent applies local quality by restricting the male sterility trait to only the cytoplasmic level (mitochondrial genome) while keeping the nuclear genome stable and uniform. The CMS trait is confined to specific mitochondrial regions, allowing the rest of the genetic composition to remain highly stable and uniform across hybrid generations.
Solution Approach 2:
The patent inverts the traditional approach by making the female parent sterile rather than the male parent. Traditionally, male sterility was used to control pollination, but this patent uses female sterility (through CMS in the female line) combined with a restorer gene in the male line to achieve controlled cross-pollination, thereby maintaining genetic stability while improving hybrid production efficiency.
3Adaptability or versatility
If multiple desirable traits are combined in a single hybrid variety, then agronomic quality and resistance improve, but breeding complexity and time required increase
Solution Approach 1:
The patent merges multiple desirable traits into a single hybrid variety by combining CMS-based self-pollination prevention with additional traits such as herbicide resistance (e.g., glyphosate resistance via EPSPS gene), insect resistance (e.g., Bt toxin genes), and improved agronomic characteristics. All these traits are integrated into one unified hybrid genotype, simplifying the breeding process while maintaining high adaptability and agronomic quality.
Solution Approach 2:
The patent creates a universal hybrid variety that performs multiple functions simultaneously: (1) prevents self-pollination through CMS, (2) provides herbicide resistance, (3) provides insect resistance, and (4) maintains high yield and agronomic quality. This multi-functional hybrid reduces the need for separate breeding programs for different traits and simplifies field management.
Data Source
AI summary
According to the invention, there is provided seed and plants of the hybrid corn variety designated CH799142. The invention thus relates to the plants, seeds and tissue cultures of the variety CH799142, and to methods for producing a corn plant produced by crossing a corn plant of variety CH799142 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH799142.