Hybrid Corn CH948868 Breeding via Cytoplasmic Male Sterility

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH948868, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility and transgenes integrated at a single chromosomal location, along with tissue cultures capable of regenerating plants with specific physiological and morphological characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to develop corn hybrids, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing uniform inbred parental lines through self-pollination, then using controlled cross-pollination only at the final hybridization stage. This segmentation allows genetic diversity to be introduced only when needed, while maintaining uniformity in the parental stock.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Inbred parental lines are developed and stabilized through multiple generations of self-pollination and selection before the hybridization step. This preliminary action ensures that the parental lines are genetically uniform and stable, which then enables predictable hybrid performance when cross-pollination occurs.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but time to achieve stable lines increases

Engineering Contradiction:
Improvegenetic uniformityVSAvoidbreeding cycle time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The breeding program uses self-pollination of inbred lines to produce hybrid seed, eliminating the need for manual emasculation and controlled pollination in the seed production phase. The male-sterile line automatically prevents self-pollination, and the maintainer line provides the pollen, allowing large-scale hybrid seed production without intensive labor.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The program changes the genetic parameter of the parental lines by introducing male-sterility through cytoplasmic genetics. This parameter change allows the female parent to naturally prevent self-pollination, replacing the need for mechanical or manual intervention and significantly reducing the time and labor required for hybrid seed production.

Inventive Principle:
Principle #35Parameter changes

3Manufacturing precision

If manual emasculation is used to prevent self-pollination, then hybrid purity is improved, but labor requirements and cost increase

Engineering Contradiction:
Improvehybrid purityVSAvoidproduction efficiency
Core Design Contradiction:
Manufacturing precisionVSEase of manufacture

Solution Approach 1:

The male-sterile line serves itself by naturally preventing self-pollination through its cytoplasmic genetics. The plant automatically produces no viable pollen, eliminating the need for manual or mechanical emasculation. This self-service mechanism maintains hybrid purity while dramatically reducing labor requirements.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The mechanical process of manual emasculation is replaced by a biological mechanism - cytoplasmic male sterility. Instead of physically removing or inactivating anthers through labor-intensive procedures, the genetic system naturally prevents pollen formation, substituting a biological effect for a mechanical operation.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

4Adaptability or versatility

If traditional breeding methods are used, then desirable traits are combined, but genetic non-uniformity and unpredictability in hybrids occur

Engineering Contradiction:
Improvetrait combinationVSAvoidhybrid performance predictability
Core Design Contradiction:
Adaptability or versatilityVSReliability

Solution Approach 1:

The breeding system segments the genetic contribution into two uniform components: a male-sterile female line and a maintainer line with desired traits. By maintaining the female line as genetically uniform through self-pollination and using it to produce all hybrids, the system ensures that variations in hybrid performance come only from the controlled introduction of male fertility genes, not from variability in the female parent.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

The female parental line is maintained as genetically homogeneous through repeated self-pollination and selection. This homogeneity in the female parent, combined with the use of uniform maintainer lines, ensures that all hybrid progeny receive identical genetic material from the female side, making hybrid performance predictable and uniform across large populations.

Inventive Principle:
Principle #33Homogeneity

Data Source

PatentUS9210895B2Plants and seeds of hybrid corn variety CH948868
Publication Date: 2015.12.15 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH948868. The invention thus relates to the plants, seeds and tissue cultures of the variety CH948868, and to methods for producing a corn plant produced by crossing a corn plant of variety CH948868 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH948868.