Hybrid Corn CH994532 Breeding Segmentation

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Solution Overview

Problem

Corn breeding techniques face challenges in developing uniform hybrid varieties with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and specific breeding methods.

Innovation Solution

The development of the hybrid corn variety CH994532, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility and genetic transformation techniques to introduce desired traits, and a method for crossing plants to produce hybrid seeds with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If cross-pollination is used to develop corn hybrids, then genetic diversity and trait combination are improved, but genetic uniformity and predictability deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSStability of the object's composition

Solution Approach 1:

The breeding process is segmented into distinct phases: first developing homozygous inbred lines through self-pollination to ensure genetic uniformity, then crossing these stabilized lines to introduce genetic diversity. This segmentation allows each phase to optimize for its specific goal without compromising the other.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Inbred lines are developed and stabilized through multiple generations of self-pollination before the actual hybrid cross. This preliminary action ensures that the parental lines are genetically uniform and stable, which then enables predictable and uniform hybrid performance when crossed.

Inventive Principle:
Principle #10Preliminary action

2Stability of the object's composition

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but genetic diversity deteriorates

Engineering Contradiction:
Improvegenetic uniformityVSAvoidgenetic diversity
Core Design Contradiction:
Stability of the object's compositionVSAdaptability or versatility

Solution Approach 1:

Self-pollination is used as a preliminary action to stabilize the genetic composition of parental lines before hybridization. This preliminary stabilization ensures uniformity in the inbred lines, which is essential for predictable hybrid performance, while the subsequent cross restores genetic diversity.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The inbred lines serve as intermediaries that carry specific desirable traits in a uniform and stable genetic background. These intermediaries are then crossed to combine multiple traits while maintaining predictability, resolving the contradiction between uniformity and diversity.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Stability of the object's composition

If multiple breeding generations are pursued to stabilize inbred lines, then uniformity and predictability are improved, but breeding time and complexity increase

Engineering Contradiction:
ImproveuniformityVSAvoidbreeding time
Core Design Contradiction:
Stability of the object's compositionVSLoss of time

Solution Approach 1:

The breeding process utilizes self-pollination, where the plant fertilizes itself, to automatically maintain genetic uniformity across generations without requiring complex external intervention. This self-service mechanism accelerates the stabilization process compared to methods requiring manual pollination and selection.

Inventive Principle:
Principle #25Self-service

Solution Approach 2:

The breeding program changes the generational parameter by advancing from F1 to F2 to F3 and subsequent generations, allowing systematic stabilization of inbred lines. This parameter change approach enables tracking and selection of uniform lines through defined generational milestones, reducing overall breeding time.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS9210851B2Plants and seeds of hybrid corn variety CH994532
Publication Date: 2015.12.15 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH994532. The invention thus relates to the plants, seeds and tissue cultures of the variety CH994532, and to methods for producing a corn plant produced by crossing a corn plant of variety CH994532 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH994532.