Hybrid Corn CH151527 CMS Pollination Control

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Solution Overview

Problem

Current corn breeding techniques face challenges in developing uniform hybrid plants with desirable traits such as high yield, disease resistance, and uniform germination due to genetic non-uniformity and unpredictability in cross-pollination, requiring the development of stable inbred plants and methods to prevent self-pollination.

Innovation Solution

The development of the hybrid corn variety CH151527, which includes genetic loci for traits like male sterility, herbicide resistance, and disease resistance, utilizing cytoplasmic-male sterility (CMS) and restorer genes to control pollination and introduce desired traits through backcrossing and genetic transformation, along with tissue culture methods to regenerate plants with specific characteristics.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Adaptability or versatility

If natural cross-pollination is used in corn breeding, then genetic diversity is increased, but genetic uniformity and predictability of hybrid performance deteriorate

Engineering Contradiction:
Improvegenetic diversityVSAvoidgenetic uniformity
Core Design Contradiction:
Adaptability or versatilityVSManufacturing precision

Solution Approach 1:

The breeding population is segmented into distinct inbred lines that are homozygous at almost all gene loci. Each inbred line is genetically uniform and true-breeding, allowing controlled crosses between specific segments to achieve desired genetic combinations while maintaining uniformity within each parent line.

Inventive Principle:
Principle #1Segmentation

Solution Approach 2:

Male sterility cytoplasm acts as an intermediary mechanism to prevent self-pollination and ensure controlled cross-pollination between specific inbred lines. The restorer gene serves as another intermediary to restore fertility in the F1 hybrid, enabling precise control over the pollination process and genetic uniformity of the hybrid population.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Manufacturing precision

If self-pollination is used to develop inbred lines, then genetic uniformity is improved, but the ability to produce uniform hybrid progeny deteriorates without controlled cross-pollination mechanisms

Engineering Contradiction:
Improvegenetic uniformityVSAvoidhybrid progeny uniformity
Core Design Contradiction:
Manufacturing precisionVSAdaptability or versatility

Solution Approach 1:

Inbred lines are developed through multiple generations of self-pollination and selection to achieve homozygosity and genetic uniformity before the cross-pollination stage. This preliminary development of uniform parental lines ensures that the subsequent F1 hybrid progeny will be uniform when crossed, as both parents have stabilized genetic compositions.

Inventive Principle:
Principle #10Preliminary action

Solution Approach 2:

The male sterility system serves as an intermediary mechanism that enables controlled cross-pollination between uniform inbred lines. By preventing self-pollination through cytoplasmic male sterility and ensuring cross-pollination only between designated parents, the system maintains both the genetic uniformity of parental lines and the uniformity of hybrid progeny.

Inventive Principle:
Principle #24Intermediary (Mediator)

3Adaptability or versatility

If multiple breeding methods are used to combine desirable traits, then trait diversity is improved, but breeding program complexity increases

Engineering Contradiction:
Improvetrait diversityVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The male sterility/restorer gene system serves multiple functions simultaneously: it prevents self-pollination, ensures controlled cross-pollination, maintains genetic uniformity of parental lines, and enables the development of uniform F1 hybrids. This multi-functional system simplifies the breeding program by consolidating several control mechanisms into a single integrated approach.

Inventive Principle:
Principle #6Universality (Multi-functionality)

Solution Approach 2:

The breeding system uses self-service mechanisms where the cytoplasmic male sterility automatically prevents self-pollination without requiring manual emasculation, and the restorer gene automatically restores fertility in the F1 hybrid. This reduces the need for labor-intensive manual interventions and simplifies the breeding process while maintaining trait diversity through controlled crosses.

Inventive Principle:
Principle #25Self-service

Data Source

PatentUS7872177B2Plants and seeds of hybrid corn variety CH151527
Publication Date: 2011.01.18 MONSANTO TECHNOLOGY LLC

AI summary

According to the invention, there is provided seed and plants of the hybrid corn variety designated CH151527. The invention thus relates to the plants, seeds and tissue cultures of the variety CH151527, and to methods for producing a corn plant produced by crossing a corn plant of variety CH151527 with itself or with another corn plant, such as a plant of another variety. The invention further relates to genetic complements of plants of variety CH151527.