Hybrid Lensless and Microscopy Imaging Device for High-Scattering Samples
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Solution Overview
Problem
Lensless imaging techniques struggle to reliably observe samples with high concentrations of scattering elements, as holograms often result in reconstruction noise and 'twin images', limiting the accuracy of cell identification and analysis in microscopy.
Innovation Solution
A device and method combining lensless imaging with a conventional microscopy mode using a magnifying optical objective, allowing for a large field of observation and precise analysis by switching between two imaging modes: a first mode for initial large-field imaging and a second mode for detailed analysis of selected regions using a second image sensor with higher magnification, and applying numerical propagation operators to reconstruct complex images from the first image.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Area of stationary object
If lensless imaging is used to observe samples, then a large field of observation is achieved, but reconstruction noise and twin images appear when scattering element concentration is high
Solution Approach 1:
The patent divides the observation process into two segments: a first imaging mode using lensless imaging for large-field overview, and a second imaging mode using conventional microscopy with a magnifying objective for detailed analysis of specific regions. This segmentation allows the system to leverage the large field of view of lensless imaging while avoiding the reconstruction noise problem by switching to conventional microscopy for high-concentration sample analysis.
Solution Approach 2:
The patent creates a multi-functional imaging device that can operate in both lensless imaging mode and conventional microscopy mode. The device includes a holder for the sample, a first light source and first image sensor for lensless imaging, and a second image sensor optically coupled to a magnifying optical objective for conventional imaging. This multi-functionality enables the system to adapt to different sample conditions by selecting the appropriate imaging mode.
2Measurement precision
If conventional microscopy with magnifying optics is used, then detailed analysis of cells is achieved, but the field of observation becomes limited
Solution Approach 1:
The patent segments the imaging function into two distinct modes: a first mode for large-field observation using lensless imaging, and a second mode for high-precision cell analysis using conventional microscopy. This segmentation allows the system to provide both broad overview capability and detailed analysis capability without compromising either function.
Solution Approach 2:
The patent adds a dimensional aspect by introducing a second imaging mode that operates in a different optical dimension (conventional microscopy with magnifying optics) compared to the first mode (lensless imaging). This dimensional addition allows the system to transition from a low-magnification overview to a high-magnification detailed view, effectively expanding the capabilities in the precision dimension while maintaining the field of view advantage from the first mode.
3Difficulty of detecting and measuring
If holographic reconstruction algorithms are applied to high-concentration samples, then cell identification is attempted, but twin images and reconstruction noise appear
Solution Approach 1:
The patent introduces an intermediary approach by using a magnifying optical objective as a mediator between the sample and the image sensor in the second imaging mode. This intermediary optical system enables detailed cell analysis without relying on holographic reconstruction algorithms that cause twin images and reconstruction noise in high-concentration samples. The magnifying objective directly focuses light onto the image sensor, providing clear images without the information loss associated with holographic reconstruction.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables reliable observation and analysis of samples with high scattering element concentrations by reducing reconstruction noise and allowing for precise identification and tracking of cells, while maintaining a large field of view.
Implementation Method 1
a first light source, able to emit an incident light wave that propagates to the sample
Implementation Method 2
diffracted waves resulting from the diffraction, by the sample, of the light wave emitted by the light source
Implementation Method 3
interference patterns generated by interference between the light wave emitted by the light source and transmitted by the sample, and diffracted waves
Implementation Method 4
a second image sensor, optically coupled to an optical system having a magnification higher than 1
Data Source
AI summary
There is provided a device allowing a sample to be observed in a first mode, by lensless imaging using a first sensor. The first mode allows a first image to be obtained, on the basis of which a region of interest of the sample may be identified. The device then allows, via a relative movement, the region of interest to be analyzed using a more precise second mode and in particular using an optical system coupled to a second sensor.