Hybrid Maize Trait Stacking for Stable Yield and Uniformity
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Solution Overview
Problem
Existing hybrid maize development methods struggle to combine desirable traits such as disease resistance, heat and drought tolerance, and improved yield while maintaining uniformity and stability in plant characteristics, which are crucial for commercial crop production.
Innovation Solution
A novel maize hybrid variety, X90P471, is developed by crossing inbred varieties and incorporating genetic loci for traits like male sterility, disease resistance, and herbicide tolerance through backcrossing and transformation, with cytoplasmically-inherited traits and regenerable tissue cultures to ensure consistent expression of desired characteristics.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If traditional plant breeding methods are used to combine multiple desirable traits, then the complexity of the breeding program increases, but the uniformity and stability of plant characteristics deteriorates
Solution Approach 1:
The patent segments the breeding process by using separate inbred parent lines (PH42YR and PH4D4P) that each carry specific desirable traits. By dividing the complex trait combination task into manageable parental components, the hybrid inherits a balanced set of traits while maintaining uniformity through controlled cross-pollination between genetically stable inbred lines.
Solution Approach 2:
The patent applies preliminary action by developing and stabilizing the inbred parent lines (PH42YR and PH4D4P) through multiple generations of self-pollination and selection before crossing. This preliminary stabilization of parental genetics ensures that the resulting hybrid F1 generation exhibits uniform and stable characteristics, as the parental lines have already been optimized for specific traits.
2Productivity
If traditional plant breeding is used to develop new varieties, then the time required for variety development increases, but the yield improvement may be limited
Solution Approach 1:
The patent utilizes preliminary action by pre-developing stable inbred parent lines (PH42YR and PH4D4P) with desired traits through extensive prior breeding work. This preliminary preparation allows the hybrid combination to quickly express high yield potential without requiring additional time-consuming breeding steps, as the parental genetic foundations are already optimized.
Solution Approach 2:
The patent merges the genetic contributions of two distinct inbred lines (PH42YR and PH4D4P) to create a hybrid that combines their respective strengths. This merging of pre-developed parental genotypes accelerates variety development by producing a high-yielding hybrid in a single generation, rather than requiring prolonged breeding cycles to achieve similar yield improvements.
3Adaptability or versatility
If multiple traits are introduced through backcrossing and transformation, then the genetic complexity of the variety increases, but the difficulty of maintaining trait stability across generations increases
Solution Approach 1:
The patent segments the introduction of multiple traits by incorporating them into separate inbred parent lines (PH42YR and PH4D4P) through controlled backcrossing and transformation events. Each parent line carries specific resistance traits in a stabilized genetic background, which simplifies the maintenance of trait stability in the hybrid compared to introducing all traits simultaneously into a single line.
Solution Approach 2:
The patent applies inversion by working backwards from the desired hybrid to identify and stabilize the necessary parental inbred lines. Rather than directly modifying the hybrid to introduce traits, the approach introduces and stabilizes traits in the parental lines first, then combines them through crossing. This reverse approach ensures trait stability is established at the parental level before hybrid formation.
Data Source
AI summary
A novel maize variety designated X90P471 and seed, plants and plant parts thereof are produced by crossing inbred maize varieties. Methods for producing a maize plant by crossing hybrid maize variety X90P471 with another maize plant are disclosed. Methods for producing a maize plant containing in its genetic material one or more traits introgressed into X90P471 through backcrossing or genetic transformation, and to the maize seed, plant and plant part produced thereby are described. Maize variety X90P471, the seed, the plant produced from the seed, and variants, mutants, and minor modifications of maize variety X90P471 are provided. Methods for producing maize varieties derived from maize variety X90P471 and methods of using maize variety X90P471 are disclosed.