Hybrid Pepper E20S0158 Breeding via Molecular Markers

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Solution Overview

Problem

There is a continuous need for new hybrid pepper cultivars that offer improved traits such as disease resistance, nutritional quality, and agronomic characteristics in the pepper (Capsicum annuum) crop, which current cultivars do not adequately address.

Innovation Solution

The development of a hybrid pepper variety 'E20S0158' with specific morphological and physiological characteristics, including resistance to certain viruses and pests, and enhanced nutritional qualities, achieved through breeding methods like backcrossing and genetic marker-enhanced selection, which can be used to produce seeds and plants with desired traits.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional breeding methods are used to develop new pepper cultivars, then genetic diversity and trait improvement are achieved, but the breeding process is time-consuming and requires multiple generations of selection

Engineering Contradiction:
Improvedisease resistanceVSAvoidbreeding cycle duration
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The patent replaces traditional mechanical breeding methods (manual crossing, visual selection, and phenotypic evaluation across multiple generations) with molecular marker-based selection. Specific DNA markers are used to identify and select plants carrying desired disease resistance genes, allowing breeders to screen for traits at the seedling stage rather than waiting for disease exposure and symptom development. This substitution of molecular tools for mechanical breeding processes dramatically reduces the time required to develop new cultivars while maintaining reliable disease resistance.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

2Adaptability or versatility

If multiple traits are selected for in pepper breeding, then overall cultivar improvement is achieved, but the complexity of the breeding program increases

Engineering Contradiction:
Improveagronomic characteristicsVSAvoidbreeding program complexity
Core Design Contradiction:
Adaptability or versatilityVSDevice complexity

Solution Approach 1:

The patent changes the parameter of selection from phenotypic traits (which require complex environmental controls and multiple evaluation criteria) to genotypic markers (DNA sequences). By selecting for specific DNA markers associated with desired traits such as disease resistance, fruit quality, and plant architecture, breeders can simultaneously track multiple traits through simple molecular assays. This parameter change from phenotypic to genotypic selection simplifies the breeding program while enabling the selection of multiple traits concurrently.

Inventive Principle:
Principle #35Parameter changes

3Measurement precision

If traditional selection methods are used to identify desirable pepper plants, then natural variation is utilized, but selection precision and efficiency are limited

Engineering Contradiction:
Improvetrait selection accuracyVSAvoidbreeding output
Core Design Contradiction:
Measurement precisionVSProductivity

Solution Approach 1:

The patent replaces traditional phenotypic selection (visual inspection, manual measurement, and subjective evaluation of plant traits) with molecular marker analysis. DNA-based markers provide objective, precise identification of plants carrying specific genes for disease resistance, fruit quality, and other desirable traits. This allows breeders to accurately identify target plants at the seedling stage without waiting for trait expression, thereby increasing both selection accuracy and breeding productivity by enabling simultaneous screening of large numbers of progeny.

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Data Source

PatentUS10595496B2Hybrid pepper ‘E2050158’
Publication Date: 2020.03.24 ENZA ZADEN BEHEER BV
  • US10595496B2 patent drawing

AI summary

A hybrid pepper designated ‘E20S0158’ is disclosed. The invention relates to the seeds of hybrid pepper ‘E20S0158’ to the plants of hybrid pepper ‘E20S0158’ and to methods for producing a hybrid plant, and to methods for producing other pepper lines, cultivars or hybrids derived from the hybrid pepper ‘E20S0158’.