Hydration Media Preserving Tissue Strength via Ion Composition
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Solution Overview
Problem
Existing methods for storing hydrated biological tissue products often result in adverse aging phenomena despite the use of isotonic and physiological pH fluids, leading to loss of intrinsic tissue properties such as elasticity, compressive strength, and shear strength.
Innovation Solution
A liquid hydration media is developed containing specific concentrations of dissolved ions, including a phosphate source and calcium silicate, to maintain and preserve biological tissue products, which can be used to rehydrate or store tissues at various temperatures, thereby retaining physical properties like elasticity, compressive strength, and shear strength.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Stability of the object's composition
If biological tissue products are stored in saline or buffered saline fluid throughout the product's shelf-life, then the tissue remains hydrated, but adverse aging phenomena occur leading to loss of intrinsic tissue properties
Solution Approach 1:
The patent modifies the chemical composition parameters of the storage medium by incorporating specific ions (calcium, phosphate, magnesium, potassium, sodium, chloride, bicarbonate) at defined concentrations. This creates a physiologically optimized solution that better mimics natural tissue environment, thereby maintaining tissue elasticity, compressive strength, and shear strength while preventing adverse aging phenomena during storage
Solution Approach 2:
The storage medium is formulated as a composite electrolyte solution combining multiple ions and buffers (including bicarbonate buffer system) rather than using simple saline. This composite composition works synergistically to maintain pH balance, osmotic pressure, and ionic composition, effectively preserving tissue properties throughout the shelf-life
2Reliability
If low temperature storage is utilized throughout the storage period to preserve intrinsic tissue properties, then tissue properties are maintained, but the storage conditions become restrictive and complex
Solution Approach 1:
The patent optimizes the chemical parameters of the storage medium (ionic composition, pH buffer capacity, osmolarity) to create a solution that provides protective effects across a broader temperature range. The bicarbonate buffer system and specific ion concentrations stabilize tissue properties whether stored at refrigeration temperatures or controlled room temperatures, reducing the stringency of temperature control requirements
3Stability of the object's composition
If dried biological tissues are reconstituted in hydration media, then the tissue becomes hydrated for use, but the process requires additional time and steps
Solution Approach 1:
The tissue is pre-treated during manufacturing to enhance its rehydration characteristics, and the storage medium is pre-formulated with optimal ionic composition and pH balance. This preliminary preparation ensures that when the tissue is placed in the hydration media, reconstitution occurs rapidly and efficiently, minimizing the time required to restore full tissue functionality before use
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The hydration media effectively preserves the physical properties and inherent growth factors of biological tissues for extended periods, maintaining up to 99% of their initial properties during storage, from minutes to several years, while allowing storage at a range of temperatures.
Implementation Method 1
The dissolved ions and physiological pH range within the hydration media act to prevent mineral leaching from biological tissues
Implementation Method 2
The method of manufacturing the liquid hydration media relies on a select combination of solutes to provide an isotonic solution and to preserve the biological tissue products
Data Source
AI summary
A hydration media for biological tissue products, methods of making the same and methods of using the same is provided. The hydration media includes sodium phosphate and calcium silicate and can be used to store or hydrate a biological tissue product.